| Apple Country Head Start at Douglas Learning Center | 598 N. Kent Street, Winchester, VA 22601 | Preschool |
| Apple Valley Montessori School | 1905 Henry Avenue, Winchester, VA 22601 | Preschool |
| Braddock Street United Methodist Early Learning Center | 115 Wolfe Street, Winchester, VA 22601 | Center |
| Building Blocks Childcare | 2170 S. Loudoun Street, Winchester, VA 22601 | Center |
| Children of America - Winchester | 631 W. Jubal Early Drive, Winchester, VA 22601 | Preschool |
| Fremont Street Nursery | 533 Fremont Street, Winchester, VA 22601 | Preschool |
| Precious One's Preschool and Daycare | 817 North Loudoun Street, Winchester, VA 22601 | Center |
| Sacred Heart Academy | 110 Keating Drive, Winchester, VA 22601 | Preschool |
| Sneakers Educational Childcare | 915 S. Cameron Street, Winchester, VA 22601 | Preschool |
| TLC Daycare Center | 32 Weems Lane, Winchester, VA 22601 | Center |
|
| The Children's Enrichment Program | 75 Merriman's Lane, Winchester, VA 22601 | Center |
| Valley Health Child Care Center | 1842 Amherst Street, Winchester, VA 22601 | Preschool |
| Winchester Academy | 2400 Roosevelt Boulevard, Winchester, VA 22601 | Preschool |
| Winchester Day Nursery | 133 Lincoln Street, Winchester, VA 22601 | Preschool |