| Alpha Christian Child Care Center | 504 Dexter Road, NW, Roanoke, VA 24019 | Center |
| Bedford YMCA at Stewartsville Elementary School | 1138 Wildcat Road, Roanoke, VA 24019 | Center |
| Bonsack Baptist Weekday Early Education Center | 4845 Cloverdale Road, Roanoke, VA 24019 | Preschool |
| Boys and Girls Clubs of Roanoke Valley - Bonsack Center | 5437 Crumpacker Drive, Roanoke, VA 24019 | Center |
| Children's Nest Learning Center - Hollins | 504 Dexter Road, Roanoke, VA 24019 | Center |
| Community School Child Care | 7815 Williamson Road NW, Hill Building, Roanoke, VA 24019 | Preschool |
| HoneyTree Early Learning Center #6 | 7507 Plantation Road, Roanoke, VA 24019 | Preschool |
| HoneytTree Early Learning Center #3 | 1530 Patrick Road, Roanoke, VA 24019 | Preschool |
| Mini-World Child Care Centers - Botetourt County | 3199 Read Mountain Road, Roanoke, VA 24019 | Preschool |
| North Star Karate | 5407 Peters Creek Rd. NW, Roanoke, VA 24019 | Preschool |