| B. W. Lester Head Start Center | 2522 Copeland Road, Valdosta, GA 31601 | Preschool |
| Bright Start Preschool Learning Center | 109 South Forrest St., Valdosta, GA 31601 | Preschool |
| Childcare Network #220 | 519 North St. Augustine Road, Valdosta, GA 31601 | Preschool |
| Children's Friend #15 | 519 St. Augustine Rd., Valdosta, GA 31601 | Preschool |
| Children's Friend #18 | 1902 N. Forrest St., Valdosta, GA 31601 | Preschool |
| Clyattville Elementary Preschool | 5386 Madison Highway, Valdosta, GA 31601 | Preschool |
| Crossing Jordan Academy | 307 Mildred St., Valdosta, GA 31601 | Center |
| Grace Day Care Center | 303 North Forrest Street, Valdosta, GA 31601 | Center |
| Hallmark Heights Head Start | 605 Hightower Street, Valdosta, GA 31601 | Preschool |
| Higher Heights Learning Center | 507 West Hill Avenue, Valdosta, GA 31601 | Center |
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| Home '2' Childcare and Development Center | 706 Broad Street, Valdosta, GA 31601 | Preschool |
| Kiddie Korral | 1611 N. Slater Street, Valdosta, GA 31601 | Center |
| Kids Cove Learning Center | 102/104 Kelly Drive, Valdosta, GA 31601 | Center |
| Lillie Bea's Day Care and Learning Center | 504 W Magnolia Street/P.O. Box 1693, Valdosta, GA 31601 | Center |
| Little Angels Pre-School & Child Care Center | 512 N. Forrest Street, Valdosta, GA 31601 | Preschool |
| Lowndes I Head Start Center | 1613 Ulmer Avenue, Valdosta, GA 31601 | Preschool |
| Oma's Learning Center & Daycare | 1345 West Hill Avenue, Valdosta, GA 31601 | Center |
| Open Arm's Daycare Center | 809 East Gordon Street, Valdosta, GA 31601 | Center |
| Tickle-Me-Pink Daycare and Learning Center | 812 West Gordon Street, Valdosta, GA 31601 | Center |
| Westside Elementary School Preschool | 2470 James Road, Valdosta, GA 31601 | Preschool |
| YMCA Prime Time | 2424 Gornto Road, Valdosta, GA 31601 | Center |
| YMCA PrimeTime at Clyattville Elementary | 5386 Madison Hwy, Valdosta, GA 31601 | Center |
| YMCA PrimeTime at Moulton-Branch Elementary | 5725 Perimeter Road, Valdosta, GA 31601 | Center |
| YMCA PrimeTime at Westside Elementary Preschool | 2470 James Road, Valdosta, GA 31601 | Center |