| Bullfrogs and Butterflies Learning Center | 1226 Sw Grandview Street, Lake City, FL 32025 | Preschool |
| Chatterbox Child Care Center | 294 Se Eloise Avenue, Lake City, FL 32025 | Preschool |
| Green Acres Learning Center | 1126 Sw Main Blvd., Lake City, FL 32025 | Center |
| Happy Hearts East Child Care Center | 149 Southeast Lochlynn Terrace, Lake City, FL 32025 | Preschool |
| Jewel's Little Rascals | 1777 Sw Leslie Glenn, Lake City, FL 32025 | Preschool |
| Kiddieland Mini-School | 168 Sw Maryland Lane, Lake City, FL 32025 | Preschool |
| Kountry Kids Day Care | 1468 South Marion Avenue, Lake City, FL 32025 | Center |
| L Pop 2 | 416 Se Ermine Ave, Lake City, FL 32025 | Center |
| Lake City Kiddy Club | 1290 S. E. Baya Drive, Lake City, FL 32025 | Preschool |
| Laura's Little Red School House | 1544 Sw Mcfarlane Avenue, Lake City, FL 32025 | Preschool |
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| Lawton's Place | 878 Ne Jackson Avenue, Lake City, FL 32025 | Center |
| Melrose Park Elementary Preschool | 820 Se Putnam Street, Lake City, FL 32025 | Preschool |