| All About God's Children Academy | 3550 Westlake Avenue, Jacksonville, FL 32206 | Preschool |
| All About Kids | 4563 Pearl Street North, Jacksonville, FL 32206 | Preschool |
| All About Kids VPK Academy | 4204 Pearl Street North, Jacksonville, FL 32206 | Center |
| Andrew Robinson Elementary Preschool | 101 West 12th Street, Jacksonville, FL 32206 | Preschool |
| Bibleway Child Care Center | 3716 N. Pearl Street, Jacksonville, FL 32206 | Center |
| Blooming Blossoms Daycare Center | 1014 East 21st Street, Jacksonville, FL 32206 | Center |
| C.U.C.I.O.M Little Disciples | 2725 North Laura Street, Jacksonville, FL 32206 | Preschool |
| Children of Liberty Child Care Center | 232 East 19th Street, Jacksonville, FL 32206 | Center |
| Cinderella Child Development Center | 44 East 27th Street, Jacksonville, FL 32206 | Preschool |
| Clayton Preschool Academy | 1419 East 15th Street, Jacksonville, FL 32206 | Preschool |
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| First Coast Young Stars Learning Center | 3911 North Main Street, Jacksonville, FL 32206 | Preschool |
| Future Duck Early Learning & Preschool | 504 Golfair Boulevard, Jacksonville, FL 32206 | Preschool |
| Future Duck Early Learning & Preschool | 619 Golfair Boulevard, Jacksonville, FL 32206 | Preschool |
| Head Start at Brentwood | 3465 Village Center Drive South, Jacksonville, FL 32206 | Preschool |
| Head Start at Jacksonville Urban League 8th St | 20 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Head Start at Northeast Springfield | 1221 E 16th Street, Jacksonville, FL 32206 | Preschool |
| Head Start at Robert F Kennedy | 1133 Ionia St, Jacksonville, FL 32206 | Preschool |
| Head Start at The Bridge | 320 West 8th Street, Jacksonville, FL 32206 | Preschool |
| Holy Rosary Catholic School | 4920 Brentwood Avenue, Jacksonville, FL 32206 | Preschool |
| John Love Elementary Preschool | 1531 Winthrop Street, Jacksonville, FL 32206 | Preschool |
| Kings and Queens Child Care and Learning Center | 3325 Plateau Street, Jacksonville, FL 32206 | Center |
| Leslie's Angels | 2114 Tilden Street, Jacksonville, FL 32206 | Center |
| Little Diamonds Big Heart Day Care | 1441 East 31st Street, Jacksonville, FL 32206 | Center |
| Long Branch Elementary Preschool | 3723 Franklin Street, Jacksonville, FL 32206 | Preschool |
| The Bridge of Northeast Florida | 320 West 8th Street, Jacksonville, FL 32206 | Center |
| The Don Brewer Early Learning Center | 1095-1 A. Philip Randolph Blvd, Jacksonville, FL 32206 | Preschool |
| Top Notch Kids Learning Center | 2726 North Main Street, Jacksonville, FL 32206 | Preschool |
| Wonderland Learning Center | 15 East 25th Street, Jacksonville, FL 32206 | Preschool |
| Young Hearts Child Development Center II | 4800 North Main Street, Jacksonville, FL 32206 | Preschool |
| Young Start Academy | 1049 Palmetto Street, Jacksonville, FL 32206 | Preschool |
| Zarephath Learning Center | 1028 East 10th Street, Jacksonville, FL 32206 | Center |