| Ann's Preschool and Kindergarten | 1777 North Meridian Road, Tallahassee, FL 32303 | Preschool |
| Astoria Park Elementary Preschool | 2465 Atlas Road, Tallahassee, FL 32303 | Preschool |
| Bainbridge Road Head Start Center | 2303 Old Bainbridge Road, Tallahassee, FL 32303 | Preschool |
| Betton Hills Preparatory School | 1815 North Meridian Road, Tallahassee, FL 32303 | Preschool |
| Bright Star Kid Care | 2920 Old Bainbridge Road, Tallahassee, FL 32303 | Preschool |
| Bright Stars Child Development Center | 2425 Spoonwood Drive, Tallahassee, FL 32303 | Preschool |
| Canopy Oaks Elementary Preschool | 3250 Pointview Drive, Tallahassee, FL 32303 | Preschool |
| Child Growth and Development | 1989 Commonwealth Lane, Tallahassee, FL 32303 | Preschool |
| Covenant Presbyterian Preschool | 2221 Old Bainbridge Road, Tallahassee, FL 32303 | Preschool |
| Faith Presbyterian Preschool and Kindergarten | 2200 North Meridian Road, Tallahassee, FL 32303 | Preschool |
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| Gingerbread Christian Academy | 3324 North Monroe Street, Tallahassee, FL 32303 | Center |
| Gingerbread Day School | 3324 North Monroe Street, Tallahassee, FL 32303 | Preschool |
| Great Beginnings Preschool | 1904 Talpeco Road, Tallahassee, FL 32303 | Preschool |
| Great Oaks Dayschool | 4840 Tower Road, Tallahassee, FL 32303 | Preschool |
| Heaven Sent Child Care Center II | 2428 Hartsfield Road, Tallahassee, FL 32303 | Preschool |
| I'm Precious Too Learning Center | 2304 Old St Augustine Road, Tallahassee, FL 32303 | Preschool |
| Imagination Day School | 3472 North Monroe Street, Tallahassee, FL 32303 | Preschool |
| Innovation School of Excellence | 2532 West Tharpe St, Tallahassee, FL 32303 | Preschool |
| Jolly Jo's Childcare Center | 1702 Gibbs Drive, Tallahassee, FL 32303 | Preschool |
| Kids Village | 1000 W Tharpe Suite 24, Tallahassee, FL 32303 | Preschool |
| Miracle Years Childcare Center I | 2403 West Tharpe Street, Tallahassee, FL 32303 | Preschool |
| Mrs V's Kidz Community College | 1212 Stone Road, Tallahassee, FL 32303 | Preschool |
| Noah Ark Christian Academy # 2 | 101 East 4th Avenue, Tallahassee, FL 32303 | Center |
| Ruediger Elementary Preschool | 526 West 10th Street, Tallahassee, FL 32303 | Preschool |
| Saint Paul's United Methodist Parents Morning Out | 1700 North Meridian Rd, Tallahassee, FL 32303 | Center |
| Tallahassee Memorial Child Care Center | 1200 East 6th Avenue, Tallahassee, FL 32303 | Preschool |
| Tallahassee Memorial Child Care Center II | 1116 East Sixth Avenue, Tallahassee, FL 32303 | Center |
| The Creative Pre-School | 2746 West Tharpe Street, Tallahassee, FL 32303 | Preschool |
| Wee Care Child Care Center | 3705 North Monroe Street, Tallahassee, FL 32303 | Preschool |