| La Petite Academy of Niceville | 4400 Highway 20 #710, Niceville, FL 32578 | Preschool |
| All God's Children Enrichment Center | 703 Powell Drive, Niceville, FL 32578 | Center |
| Child Development and Education Center | 100 College Boulevard Building M, Niceville, FL 32578 | Preschool |
| Discovery Learning Academy | 4565 Commercial Drive, Niceville, FL 32578 | Preschool |
| First Baptist Church of Niceville Child Development Center | 622 Bayshore Drive, Niceville, FL 32578 | Preschool |
| First United Methodist Church Wesley Academy Preschool | 214 South Partin Drive, Niceville, FL 32578 | Preschool |
| Happyland Preschool | 1018 White Point Road, Niceville, FL 32578 | Preschool |
| Niceville Head Start Center | 1200 Valparaiso Boulevard, Niceville, FL 32578 | Preschool |
| Pam's Lighthouse Learning Center | 1209 47th Street, Niceville, FL 32578 | Preschool |
| Rocky Bayou Christian School | 2101 North Partin Drive, Niceville, FL 32578 | Preschool |
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| St Paul Lutheran Preschool | 1407 E John Sims Pkwy, Niceville, FL 32578 | Preschool |
| The Montessori School for the Arts | 410 Fir Avenue, Niceville, FL 32578 | Preschool |
| The River Academy | 100-a Hart Street, Niceville, FL 32578 | Preschool |