| A Child's Delight at Eden Park | 1330 Ne 39th Avenue, Gainesville, FL 32609 | Center |
| A Child's Frontier | 3520 Nw 13th Street, Gainesville, FL 32609 | Center |
| A World of Angels of Gainesville | 1119 Nw 42nd Avenue, Gainesville, FL 32609 | Preschool |
| A&M 4 Kids | 2207 Nw 10th Street, Gainesville, FL 32609 | Preschool |
| Alachua County Child Care Center | 4138 Nw 13th Street, Gainesville, FL 32609 | Preschool |
| Hand N Hand Christian Child Care Center | 1936 Nw 6th Street, Gainesville, FL 32609 | Center |
| Heavenly Kids Academy | 309 Ne 39th Avenue, Gainesville, FL 32609 | Preschool |
| Lil' Doves Christian Day Care | 4232 Nw 6th Street Bldg 2, Gainesville, FL 32609 | Center |
| Pinewood School | 1704 Ne 9th Street, Gainesville, FL 32609 | Center |
| SBAC - Metcalfe Elementary School Preschool | 1250 Ne 18 Avenue, Gainesville, FL 32609 | Preschool |
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| SBAC - Rawlings Elementary School Preschool | 3500 Me 15 Street, Gainesville, FL 32609 | Preschool |
| Smokey Bear Kiddy College | 2500 Ne 15th Street, Gainesville, FL 32609 | Preschool |