| 4-C Headstart - Midway | 2255 Rightway Street, Sanford, FL 32771 | Center |
| Academic Beginnings Child Care | 315 Maple Avenue, Sanford, FL 32771 | Preschool |
| All God's Children Preschool | 240 San Marcos Avenue, Sanford, FL 32771 | Center |
| Bee Kids Academy | 2581 S. Sanford Avenue, Sanford, FL 32771 | Preschool |
| Bee Kids Academy Too | 1104 West 3rd Street, Sanford, FL 32771 | Center |
| Brilliant Minds Montessori | 153 Bellagio Circle, Sanford, FL 32771 | Center |
| Coqui Academy | 2401 South Park Avenue, Sanford, FL 32771 | Preschool |
| Dr Sharon Riggins Patterson Child Development Center | 2400 Chase Avenue, Sanford, FL 32771 | Preschool |
| First Baptist Sweetwater Mother's Day Out | 3800 Wekiva Springs Road, Sanford, FL 32771 | Preschool |
| First United Methodist Preschool & Childcare Center | 419 Park Avenue, Sanford, FL 32771 | Preschool |
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| Hidden Treasures Pre School and Child Care Center | 1018 West First Street, Sanford, FL 32771 | Preschool |
| Holy Cross Lutheran Child care Center | 5450 Holy Cross Court, Sanford, FL 32771 | Preschool |
| Idyllwilde Elementary Vpk - Seminole County | 430 Vihlen Road, Sanford, FL 32771 | Preschool |
| Kid City USA - Sanford | 2720 W 25th Street, Sanford, FL 32771 | Preschool |
| Kiddy Cats Academy | 360 S Mellonville Ave, Sanford, FL 32771 | Preschool |
| Ladybird Academy | 700 Lake Forest Boulevard, Sanford, FL 32771 | Preschool |
| Lakeside Fellowship UMC Preschool | 305 South Orange Boulevard, Sanford, FL 32771 | Preschool |
| Little Acorns Child Care - Sanford | 630 Riverview Avenue, Sanford, FL 32771 | Preschool |
| Merthie's Learning Center | 1611 Merthie Dr, Sanford, FL 32771 | Preschool |
| New Path Montessori School | 301 San Marcos Avenue Suite 300, Sanford, FL 32771 | Preschool |
| Page Private School | 100 Aero Lane, Sanford, FL 32771 | Center |
| Park Avenue Headstart | 301 South Oak Avenue, Sanford, FL 32771 | Center |
| Pine Crest Elementary Vpk | 405 W. 27th Street, Sanford, FL 32771 | Preschool |
| Primrose School of Lake Mary Heathrow | 1200 Orange Blvd., Sanford, FL 32771 | Center |
| Rescue Day Care | 1315 South Summerlin Ave, Sanford, FL 32771 | Preschool |
| River Walk Christian Child Care | 801 W . 22nd Street, Sanford, FL 32771 | Center |
| Sanford Child Development | 1320 Hickory Ave, Sanford, FL 32771 | Preschool |
| Sanford Early Childhood Center | 801 East 25th Street, Sanford, FL 32771 | Preschool |
| Seminole High School Vpk | 2701 Ridgewood Avenue, Sanford, FL 32771 | Preschool |
| Wee The People Preschool and Child Care | 708 S Elm Ave, Sanford, FL 32771 | Preschool |