| ABC Learning Tree | 2778 South Westmoreland Drive, Orlando, FL 32805 | Preschool |
| Academy 2000 | 624 Bethune Drive, Orlando, FL 32805 | Preschool |
| Arcoiris De Amor Child Care | 929 W. Michigan Street, Orlando, FL 32805 | Center |
| Arising Little Angels Preschool | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Arising Little Angels Preschool Academy | 1225 West 25th Street, Orlando, FL 32805 | Preschool |
| Catalina Elementary School Preschool | 2448 W. 29th Street, Orlando, FL 32805 | Preschool |
| Children's Palace | 1800 W Washington St, Orlando, FL 32805 | Center |
| Early Learning Center at First Orlando | 3000 S. John Young Pkwy, Orlando, FL 32805 | Center |
| Frontline Head Start Center | 3000 County Road Smith Street, Orlando, FL 32805 | Preschool |
| Frontline Outreach Child Development Center | 3000 Cr Smith Street, Orlando, FL 32805 | Preschool |
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| Grand Avenue Elementary School Preschool | 800 W. Grand St., Orlando, FL 32805 | Preschool |
| Greater Tomorrow Christian Academy | 16 South Dollins Avenue, Orlando, FL 32805 | Preschool |
| Happy Times Learning Academy | 1215 West Gore Street, Orlando, FL 32805 | Center |
| Kid's Kingdom Daycare and Christian Academy | 1342 Bruton Blvd, Orlando, FL 32805 | Preschool |
| Kids Kingdom Christian Academy | 1342 Bruton Blvd., Orlando, FL 32805 | Preschool |
| Kids Time Kiddie Care | 197 South Cottage Hill Road, Orlando, FL 32805 | Center |
| Kidz at Work | 2035 West Washington Street, Orlando, FL 32805 | Preschool |
| Little Angels Christian Academy | 1340 West Michigan Street, Orlando, FL 32805 | Preschool |
| Little Creations Child Care | 2740 W. Washington St, Orlando, FL 32805 | Preschool |
| Little Miracles Academy | 900 Plymouth Avenue, Orlando, FL 32805 | Center |
| Little People Learning Center | 1033 W Michigan St, Orlando, FL 32805 | Preschool |
| Little Rae's Christian Center | 3099 Orange Center Boulevard, Orlando, FL 32805 | Preschool |
| Margie's Non-Stop Kiddie Kare | 903 Plymouth Avenue, Orlando, FL 32805 | Preschool |
| Mt Sinai Child Development Center | 2610 Orange Center Blvd, Orlando, FL 32805 | Preschool |
| Peabo Child Care Center | 815 Hills Street, Orlando, FL 32805 | Center |
| Peter Pan Child Care Center | 1602 Bruton Boulevard, Orlando, FL 32805 | Center |
| Pineloch Elementary School Preschool | 3101 Woods Ave, Orlando, FL 32805 | Preschool |
| Raising Angels Child Care | 2385 West Church Street, Orlando, FL 32805 | Center |
| Raising Angels Child Development Center | 1720 Rio Grande Avenue, Orlando, FL 32805 | Center |
| Rock Lake Elementary School Preschool | 408 N. Tampa Ave, Orlando, FL 32805 | Preschool |
| TLC Christian Academy | 1408 W Michigan Street, Orlando, FL 32805 | Preschool |
| The Garden Early Care and Learning Center | 1 South Cottage Hill Road, Orlando, FL 32805 | Preschool |
| The Little Red Train Pre-School and Child Care | 647 W Indiana St, Orlando, FL 32805 | Preschool |
| Tomorrow's Academy | 3121 Monte Carlo Trail, Orlando, FL 32805 | Center |
| Tots in Toyland Learning Center | 11235 West Michigan Street, Orlando, FL 32805 | Preschool |
| Washington Shores Elementary School Preschool | 944 West Lake Mann Drive, Orlando, FL 32805 | Preschool |