| A + Learning Center | 221 W. Alexander Street, Plant City, FL 33563 | Preschool |
| Children's Nest Day School | 1311 Mendonsa Road, Plant City, FL 33563 | Preschool |
| Easter Seals Child Development at Teen Parent East | 1202 Grant Street, Plant City, FL 33563 | Center |
| First Presbyterian Learning Center | 404 W. Reynolds Street, Plant City, FL 33563 | Center |
| Fun Factory Preschool | 410 S. Collins Street, Plant City, FL 33563 | Preschool |
| Gospel Tabernacle Learning Center | 801 Wilkins Avenue, Plant City, FL 33563 | Center |
| Kids Club Early Learning Center | 1108 Goldfinch Drive, Plant City, FL 33563 | Center |
| Kimberly's Infant Care Center | 605 S. Drane Street, Plant City, FL 33563 | Preschool |
| Linda's Little People | 1406 N. Wheeler Street, Plant City, FL 33563 | Center |
| Little Explorer's Learning Academy | 301 E. Alsobrook Street, Plant City, FL 33563 | Center |
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| Little Munchkins | 702 S. Ever Street, Plant City, FL 33563 | Center |
| Plant City Head Start Center | 1304 Alsobrook Street, Plant City, FL 33563 | Preschool |
| Plant City High Little School | 1 Raider Place, Plant City, FL 33563 | Preschool |
| Plant City Learning Center | 651 E. Alexander Street, Plant City, FL 33563 | Center |
| RCMA Central Plant City Child Development Center | 508 Lakewood Avenue, Plant City, FL 33563 | Preschool |
| RCMA Westside Village Child Development Center | 3102 Sammonds Road, Plant City, FL 33563 | Preschool |
| Shiloh Baptist Church Preschool | 905 W. Terrace Street, Plant City, FL 33563 | Center |
| Smart Kids Academy | 3512 W. Baker Street, Plant City, FL 33563 | Preschool |
| St. Clement Early Childhood Center | 1104 N. Alexander Street, Plant City, FL 33563 | Preschool |
| Strawberry Patch | 2805 W. Reynolds Street, Plant City, FL 33563 | Center |
| Tots Active Learning Center | 121 W. Prosser Drive, Plant City, FL 33563 | Center |