| Brenda's Little Helpers Christian Daycare | 1100 E Rose Street, Lakeland, FL 33801 | Center |
| CT&S Annointed Learning Center II | 2302 Haywood Street, Lakeland, FL 33801 | Preschool |
| Creative Minds Academy II | 1802 Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Creative Minds Learning Academy | 3108 Atlantic Ave, Lakeland, FL 33801 | Center |
| Early Childhood Learning Center | 1703 Skinner Street, Lakeland, FL 33801 | Preschool |
| First United Methodist Church Preschool | 72 Lake Morton Drive, Lakeland, FL 33801 | Preschool |
| Florida Southern College Preschool Lab | 111 Lake Hollingsworth Dr, Lakeland, FL 33801 | Center |
| Future Children Development Center | 2128 S Crystal Lake Dr, Lakeland, FL 33801 | Center |
| God Little Helpers | 1641 E Memorial Blvd, Lakeland, FL 33801 | Center |
| Grace Lutheran Child Development Center | 745 South Ingraham Avenue, Lakeland, FL 33801 | Preschool |
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| Heavenly Kids Christian Academy | 2605 Sunshine Drive South, Lakeland, FL 33801 | Center |
| JC's Early Learning Center | 2540 S Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Kids Town Preschool | 333 Griffin Avenue, Lakeland, FL 33801 | Preschool |
| Lakeland Montessori Schoolhouse | 837 E. Parker Street, Lakeland, FL 33801 | Preschool |
| Lighthouse Community Preschool | 215 East Magnolia St, Lakeland, FL 33801 | Center |
| Little Shepherds Preschool | 175 Lake Hollingsworth Drive, Lakeland, FL 33801 | Center |
| Lonnie's Little Professors Childcare Center | 905 E Parker St, Lakeland, FL 33801 | Center |
| McCloud's Childcare Center | 1741 E Gary Road, Lakeland, FL 33801 | Preschool |
| SACCA Inc Philip O'Brien Campus Kid Care | 1225 E Lime St, Lakeland, FL 33801 | Center |
| Sister's Learning Enrichment Center | 850 N Lake Avenue, Lakeland, FL 33801 | Center |
| Sonrise Christian Learning Center | 3151 Hardin Combee Rd, Lakeland, FL 33801 | Center |
| The Salvation Army HALO Child Enrichment Center | 835 N Kentucky Ave, Lakeland, FL 33801 | Center |
| Treehouse Learning & Enrichment Center | 2025 Bartow Rd., Lakeland, FL 33801 | Preschool |
| Treehouse Learning Center | 2025 Bartow Road, Lakeland, FL 33801 | Center |