| 21st Century Homework Club | 907 Kingsprings Road, Johnson City, TN 37601 | Center |
| Carver Head Start | 500 Washington St, Johnson City, TN 37601 | Center |
| Central Baptist Preschool | 300 North Roan Street, Johnson City, TN 37601 | Preschool |
| Central Elementary - Head Start | 252 Taylor Town Rd., Johnson City, TN 37601 | Preschool |
| Central Elementary Carter Cares | 252 Taylortown Road, Johnson City, TN 37601 | Center |
| Cherokee Christian Learning Center | 2615 South Roan Street, Johnson City, TN 37601 | Preschool |
| Children First Head Start Center | 837 Pardee St., Johnson City, TN 37601 | Preschool |
| Coalition For Kid's - Kid's In Action | 2308 Watauga Rd., Johnson City, TN 37601 | Center |
| Coalition For Kids Mountain View Elementary | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Coalition For Kids North Side Elementary | 1000 N. Roan St., Johnson City, TN 37601 | Center |
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| Fairmont Elementary - Educare | 1405 Lester Harris Road, Johnson City, TN 37601 | Center |
| First Christian Church Preschool | 200 Mountcastle Drive, Johnson City, TN 37601 | Preschool |
| Happy Valley Elementary Head Start | 1840 Milligan Highway, Johnson City, TN 37601 | Preschool |
| Happy Valley Elementary School - Extended School Program | 1840 Milligan Highway, Johnson City, TN 37601 | Center |
| Indian Trail Intermediate School Educare | 307 Car-mol Drive, Johnson City, TN 37601 | Center |
| Johnson City Prek At Fairmont Elementary | 1405 Lester Harris Road, Johnson City, TN 37601 | Preschool |
| Lake Ridge Elementary - Educare | 1001 Lake Ridge Square, Johnson City, TN 37601 | Center |
| Lake Ridge Elementary - Preschool | 1001 Lake Ridge Square, Johnson City, TN 37601 | Preschool |
| Lake Terrace Head Start | 2450 Mint Hill Rd, Johnson City, TN 37601 | Preschool |
| Mountain View Elementary Educare | 907 King Springs Road, Johnson City, TN 37601 | Center |
| Munsey Memorial Preschool | 201 E. Market St., Johnson City, TN 37601 | Preschool |
| North Side Elementary School Educare | 1000 North Roan Street, Johnson City, TN 37601 | Center |
| Northside Elementary Pre-kindergarten | 1000 N. Roan, Johnson City, TN 37601 | Preschool |
| Pinecrest Head Start | 500 Angel Drive, Johnson City, TN 37601 | Preschool |
| Playstation - The Kids Depot | 1006 South Broadway, Johnson City, TN 37601 | Center |
| Princeton Preporatory | 504-a Princeton Road, Johnson City, TN 37601 | Center |
| Rise Up! | 1500 East Millard Street, Johnson City, TN 37601 | Center |
| Science Hill Preschool | Technology Center, Johnson City, TN 37601 | Preschool |
| Small Steps Children's Academy | 1201 East Chilhowie Ave., Johnson City, TN 37601 | Center |
| St. Mary's School | 2211 East Lakeview Drive, Johnson City, TN 37601 | Center |
| Stepping Stones Day Care And Preschool | 216 East Market St., Johnson City, TN 37601 | Preschool |
| Wesley Memorial United Methodist Church Preschool | 225 Princeton Road, Johnson City, TN 37601 | Preschool |