| Harmony Head Start Center | 1019 Painter Road, Jonesborough, TN 37659 | Preschool |
| Jonesborough Elementary - SACC | 306 Forest Drive, Jonesborough, TN 37659 | Center |
| Jonesborough Middle School SACC | 308 Forest Drive, Jonesborough, TN 37659 | Center |
| Lamar Elementary - SACC | 3261 Highway 81s, Jonesborough, TN 37659 | Center |
| Lamar Elementary Pre-kindergarten | 3261 Highway 81 South, Jonesborough, TN 37659 | Preschool |
| Learn And Grow Childcare Center | 604 Boones Creek Road, Jonesborough, TN 37659 | Center |
| Little Bo Peep Learning Center | 305 West Main, Jonesborough, TN 37659 | Center |
| Little Tigers Preschool & Enrichment | 305 Forest Dr, Jonesborough, TN 37659 | Preschool |
| Persimmon Ridge Head Start | 1521 Persimmon Ridge Rd, Jonesborough, TN 37659 | Preschool |
| Sulphur Springs Community Child Care | 1432 Gray-sulphur Springs Rd., Jonesborough, TN 37659 | Center |
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| Sulphur Springs School - SACC | 1518 Gray Station/sulphur Springs Rd., Jonesborough, TN 37659 | Center |