| A Alphabet Academy Child Care Center | 1803 West Broadway Avenue, Louisville, KY 40203 | Preschool |
| Angels of Myricles | 1225 West Jefferson Street, Louisville, KY 40203 | Preschool |
| Aunt Gigi's Little Jewels Day Care Center | 748 South Floyd Street, Louisville, KY 40203 | Preschool |
| Caterpillar Clubhouse | 330 East Oak Street, Louisville, KY 40203 | Preschool |
| Chestnut Street YMCA Child Development | 930 West Chestnut Street, Louisville, KY 40203 | Preschool |
| Children's Choice Learning Center | 204 East Jacob Street, Louisville, KY 40203 | Preschool |
| Coleridge-Taylor Elementary Childcare Enrichment Program | 1130 West Chestnut Street, Louisville, KY 40203 | Center |
| Community Childcare Center #2 | 517 West Breckinridge Street, Louisville, KY 40203 | Preschool |
| Dawson Orman Education Center | 900 South Floyd Street, Louisville, KY 40203 | Preschool |
| Faith Academy | 1230 South 3rd Street, Louisville, KY 40203 | Preschool |
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| Granny's Angels | 334 East Oak Street, Louisville, KY 40203 | Preschool |
| Jefferson Community and Technical College Early Childhood Development Center | 800 West Chestnut Street, Louisville, KY 40203 | Preschool |
| Kids First Academy III | 529 North 22nd Street, Louisville, KY 40203 | Preschool |
| Lil' Genius Child Care Center | 1121 West Broadway, Louisville, KY 40203 | Preschool |
| Love and Prayer Childcare | 1933 West Madison Street, Louisville, KY 40203 | Preschool |
| Mary E Spradling Day Nursery | 726 South Preston Street, Louisville, KY 40203 | Preschool |
| Mini-Versity Downtown Child Development Center | 306 Roy Wilkins Avenue, Louisville, KY 40203 | Preschool |
| Presbyterian Child Development Center | 630 Finzer Street, Louisville, KY 40203 | Preschool |
| Southside Christian Day Care V | 817 South Second Street, Louisville, KY 40203 | Preschool |
| The Learning Tree Child Development Center | 1912 W. Broadway Street, Louisville, KY 40203 | Preschool |
| The Wright Place Day Care Center | 600 South 18th Street, Louisville, KY 40203 | Preschool |