| A Child's Place | 107 Brookside Drive, Nicholasville, KY 40356 | Preschool |
| Academy of Little Learners | 812 Wilmore Road Ste.600, Nicholasville, KY 40356 | Preschool |
| All God's Children Day Care | 122 Danville Road Loop 1, Nicholasville, KY 40356 | Preschool |
| Bellaire Creative Christian Academy | 220 Bellaire Drive, Nicholasville, KY 40356 | Preschool |
| Bethel Early Learning Center | 3260 Lexington Road, Nicholasville, KY 40356 | Preschool |
| Brookside Elementary- YMCA of Central KY | 199 Brookside Drive, Nicholasville, KY 40356 | Center |
| Childcare Network #257 | 1101 Lauderdale Dr., Nicholasville, KY 40356 | Preschool |
| Children's Choice Learning Center | 717 S. Main St, Nicholasville, KY 40356 | Preschool |
| Children's Creative Campus | 700 South Main Street, Nicholasville, KY 40356 | Preschool |
| Creation Kingdom-Brannon Crossing | 1101 Lauderdale Dr, Nicholasville, KY 40356 | Preschool |
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| Creative Montessori School | 3220 Lexington Road, Nicholasville, KY 40356 | Preschool |
| Garden Gate Preschool | 101 North First Street, Nicholasville, KY 40356 | Preschool |
| Imagination Station Learning Center | 305 W. Chestnut St., Nicholasville, KY 40356 | Preschool |
| Jessamine Montessori | 150 Courchelle Drive, Nicholasville, KY 40356 | Preschool |
| Kid's Connection Learning Center | 310 Southview Drive, Nicholasville, KY 40356 | Preschool |
| Kiddie Kottage | 101 Depot Street, Nicholasville, KY 40356 | Preschool |
| Learning Tree Montessori | 706 North Main Street, Nicholasville, KY 40356 | Preschool |
| Lexington Christian Academy Providence Campus | 101 Providence Way, Nicholasville, KY 40356 | Preschool |
| Nana's Play & Learn Village | 3041 Elizabeth Street, Nicholasville, KY 40356 | Preschool |
| Nicholasville Elementary YMCA Primetime | 414 West Maple Street, Nicholasville, KY 40356 | Center |
| Our House Creative Child Care | 104 Mint Lane, Nicholasville, KY 40356 | Preschool |
| Rosenwald - Dunbar Elementary YMCA Prime Time | 1500 Wilmore Road, Nicholasville, KY 40356 | Preschool |
| The Learning Jungle Childcare Center | 981 S. Main Street, Nicholasville, KY 40356 | Preschool |
| YMCA Afterschool at the Jessamine Early Learning Village | 851 Wilmore Road, Nicholasville, KY 40356 | Center |
| YMCA Warner After School Care | 821 Wilmore Road, Nicholasville, KY 40356 | Center |