| La Petite Academy of Lexington | 148 Malabu Drive, Suite 110, Lexington, KY 40503 | Preschool |
| Lexington KinderCare | 2611 Wilhite Dr, Lexington, KY 40503 | Preschool |
| Wellington KinderCare | 628 Wellington Way, Lexington, KY 40503 | Preschool |
| Barbara's Loving Learning Center | 3323 Partner Place, Lexington, KY 40503 | Preschool |
| Central Baptist Hospital Child Development Center | 110 Southland Drive, Lexington, KY 40503 | Preschool |
| Cherish The Child Care Center | 102 Dennis Drive 107 Dennis Drive, Lexington, KY 40503 | Preschool |
| Child Development Center of the Bluegrass | 290 Alumni Drive, Lexington, KY 40503 | Preschool |
| Community Montessori School | 166 Crestwood Drive, Lexington, KY 40503 | Preschool |
| Creative Kids Child Care Center | 2385 Huguenard Drive, Lexington, KY 40503 | Preschool |
| Crestwood Christian Church Childcare Center | 1882 Bellefonte Drive, Lexington, KY 40503 | Preschool |
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| Extended School Program at Stonewall Elementary | 3215 Cornwall Drive, Lexington, KY 40503 | Center |
| Extended School Program at Wellington Elementary | 3280 Keithshire Way, Lexington, KY 40503 | Center |
| Fayette Co-Operating Nursery School & Kindergarten | 109 Rosemont Garden, Lexington, KY 40503 | Preschool |
| Fayette Cooperating Nursery School | 3564 Clays Mill Road, Lexington, KY 40503 | Preschool |
| Lafayette Christian Church Child Care | 1836 & 1824 Clays Mill Road, Lexington, KY 40503 | Preschool |
| Mary Queen of the Holy Rosary Preschool and After Care | 605 Hill-N-Dale Drive, Lexington, KY 40503 | Preschool |
| Miracles and Wonders Play and Learn Center | 3330 Partner Place #130, Lexington, KY 40503 | Preschool |
| Pathway Christian Learning Center | 2601 Clays Mill Road, Lexington, KY 40503 | Preschool |
| Pepperhill Day School | 2104 Eastway Drive, Lexington, KY 40503 | Preschool |
| Southern Hills Early Childhood Program | 2356 Harrodsburg Road, Lexington, KY 40503 | Preschool |
| Southern Hills Early Learning Lab | 2356 Harrodsburg Road, Lexington, KY 40503 | Preschool |
| Southern Hills Montessori School | 2356 Harrodsburg Road, Lexington, KY 40503 | Preschool |
| Southland Kids Kare | 2094 Harrodsburg Road, Lexington, KY 40503 | Preschool |
| St. Michael's School | 2025 Bellefonte Drive, Lexington, KY 40503 | Preschool |
| Summit Christian Preschool | 2780 Clays Mill Road, Lexington, KY 40503 | Preschool |
| Tots Landing Learning Center | 3501 Beaver Place Road, Lexington, KY 40503 | Preschool |
| YMCA After School at Glendover Elementary | 710 Glendover Road, Lexington, KY 40503 | Center |
| YMCA Clays Mill Elementary | 2319 Clays Mill Road, Lexington, KY 40503 | Center |
| YMCA at Rose Campus LCA | 450 W. Reynolds Rd., Lexington, KY 40503 | Center |