| Dovie Thompson Child Development Center | 207 2nd Street, Barbourville, KY 40906 | Preschool |
| Henson Hollow Child Development Center | 188 Henson Hollow Road, Barbourville, KY 40906 | Preschool |
| Jack-N-Jill Daycare | 401 Cumberland Avenue, Barbourville, KY 40906 | Preschool |
| KCEOC St. Gregory Head Start Center | 329 Sycamore Street, Barbourville, KY 40906 | Preschool |
| Kids Being Kids | 202 25 East, Barbourville, KY 40906 | Preschool |
| Kids First Learning Child Care Center | 103 Winchester Street, Barbourville, KY 40906 | Preschool |
| Knox Central Teen Parenting Center | 100 Panther Way, Barbourville, KY 40906 | Preschool |
| Rosenwald Child Development Center | Whitesburg Hollow - Boone Heights, Barbourville, KY 40906 | Preschool |
| Tarry -Here Daycare | 2047 Ky 459, Barbourville, KY 40906 | Preschool |
| The Children's House Day Care | 205 Manchester Street, Barbourville, KY 40906 | Preschool |
|
| Tiny Tot's Childcare | 595 Ky Hwy 3439 Old Us 25e, Barbourville, KY 40906 | Preschool |