| A Kid's Place | 500 North Main Street, Hopkinsville, KY 42240 | Preschool |
| Belmont's Center of Sunshine & Rainbows | 814 Belmont Street, Hopkinsville, KY 42240 | Center |
| Christian County Head Start | 1355 Glass Avenue, Hopkinsville, KY 42240 | Preschool |
| First United Methodist Church Daycare | 1305 South Main Street, Hopkinsville, KY 42240 | Preschool |
| Heritage Christian Academy | 8349 Eagle Way Bypass, Hopkinsville, KY 42240 | Preschool |
| Holiday Elementary Child Care Services | 3910 Nassau Circle, Hopkinsville, KY 42240 | Center |
| Hopkinsville Child Development Center | 2511 Fort Campbell Blvd., Hopkinsville, KY 42240 | Preschool |
| House Of Treasures | 196 Mitch Lane, Hopkinsville, KY 42240 | Preschool |
| House of Angels | 1015 Skyline Drive, Hopkinsville, KY 42240 | Preschool |
| Indian Hills Elementary Child Care Services | 313 Blane Drive, Hopkinsville, KY 42240 | Preschool |
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| It's A Small World Child Care Center | 621 Millbrooke Drive, Hopkinsville, KY 42240 | Preschool |
| Kidz Academy | 1027 Pyle Lane, Hopkinsville, KY 42240 | Preschool |
| Let's Go Play "Academy" | 15 Airport Road, Hopkinsville, KY 42240 | Preschool |
| Little Angels Daycare Center | 406 North Main Street, Hopkinsville, KY 42240 | Preschool |
| Martin Luther King Jr. Elementary Enrichment Center | 14405 Dr. Martin Luther King Jr. Way, Hopkinsville, KY 42240 | Center |
| Millbrooke Elementary Child Care Services | 415 Millbrooke Drive, Hopkinsville, KY 42240 | Center |
| New Life Ministries Child Care Center | 202 Trail Of Tears, Hopkinsville, KY 42240 | Preschool |
| Perfect Loving Care | 180 & 190 Cave Street, Hopkinsville, KY 42240 | Preschool |
| Promise Land Preschool | 2601 S. Walnut Street, Hopkinsville, KY 42240 | Preschool |
| Raggedy Ann & Andy Child Development Center | 189 Apache Drive & 105 Navajo Trail, Hopkinsville, KY 42240 | Preschool |
| Shepherd's Little Lamb's Day Care | 212 Sanderson Drive, Hopkinsville, KY 42240 | Preschool |
| Sinking Fork Elementary Child Care Services | 5005 Princeton Road, Hopkinsville, KY 42240 | Center |
| Small Steps of Faith Childcare | 620 North Drive, Hopkinsville, KY 42240 | Preschool |
| The Children's Academy of Hopkinsville | 120 Iroquois Drive, Hopkinsville, KY 42240 | Preschool |
| University Heights Academy Preschool | 1300 Academy Drive, Hopkinsville, KY 42240 | Preschool |
| Visions Child Development Center | 2204 Canton Street, Hopkinsville, KY 42240 | Preschool |
| Waddell's Play & Learn Child Care Center | 403 Moores Drive, Hopkinsville, KY 42240 | Preschool |
| Weekday Ministries Program | 1400 South Main Street, Hopkinsville, KY 42240 | Preschool |