| 'Trib Ball "Free to Learn" Daycare' | 801 West 5th Street, Owensboro, KY 42301 | Preschool |
| Academy of Learning | 730 Harvard Drive, Owensboro, KY 42301 | Preschool |
| Academy of Learning | 4801 Towne Square, Owensboro, KY 42301 | Preschool |
| After Care Program OMS South | 2631 South Griffith Avenue, Owensboro, KY 42301 | Center |
| Audubon Elementary After School Program | 300 Worthington Road, Owensboro, KY 42301 | Preschool |
| Audubon Elementary Head Start | 300 Worthington Road, Owensboro, KY 42301 | Preschool |
| Beginning Impressions Child Development Center | 4225 Benttree Drive, Owensboro, KY 42301 | Preschool |
| Burns Elementary After School Program | 4514 Goetz Drive, Owensboro, KY 42301 | Center |
| Cathedral Preschool | 600 Locust Street, Owensboro, KY 42301 | Preschool |
| Hastings' Early Learning School | 1945 Tamarack Road, Owensboro, KY 42301 | Preschool |
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| Heritage Park Preschool and Nursery | 3802 Legacy Run, Owensboro, KY 42301 | Preschool |
| Killian Center for Migrant Head Start Services | 900 Walnut Street, Owensboro, KY 42301 | Preschool |
| Our Lady of Lourdes Day Care | 4005 Frederica Street, Owensboro, KY 42301 | Preschool |
| Over The Rainbow Childcare & Preschool | 411 West Fourth Street, Owensboro, KY 42301 | Preschool |
| Owensboro Family YMCA Afterschool & Preschool | 900 Kentucky Parkway, Owensboro, KY 42301 | Preschool |
| Peace Lutheran Preschool | 2200 Carter Road, Owensboro, KY 42301 | Preschool |
| Pleasant Grove Christian Learning Center | 5664 State Route 56, Owensboro, KY 42301 | Preschool |
| Precious Blood Day Care | 3400 Fenmore Street, Owensboro, KY 42301 | Preschool |
| Rose's Day Care | 2821 West 5th Street, Owensboro, KY 42301 | Preschool |
| Sorgho Elementary After School Program | 5390 Kentucky Highway 56, Owensboro, KY 42301 | Preschool |
| Sutton Elementary School Childcare Program | 2060 Lewis Lane, Owensboro, KY 42301 | Center |
| Tamarack Elementary After School Program | 1733 Tamarack Road, Owensboro, KY 42301 | Center |
| Tender Loving Care Child Development Center | 1528 West 9th Street, Owensboro, KY 42301 | Preschool |
| West End Family Development Center | 800 West 5th Street, Owensboro, KY 42301 | Preschool |
| West Louisville Elementary After School Program | 8400 Ky 56, Owensboro, KY 42301 | Center |