| Academy of Learning | 3220 Bold Forbes Way, Owensboro, KY 42303 | Preschool |
| Animal Crackers Nursery & Day School | 1304 St. Ann Street, Owensboro, KY 42303 | Preschool |
| Buena Vista Christian Learning Center | 100 West 23rd Street, Owensboro, KY 42303 | Preschool |
| Country Heights After School Program | 4961 Ky Highway 54, Owensboro, KY 42303 | Center |
| Dar-Nek Family Development Center | 4800 New Hartford Road, Owensboro, KY 42303 | Preschool |
| Deer Park After School Program | 4959 New Hartford Road, Owensboro, KY 42303 | Center |
| East End Angels Daycare | 5533 Highway 144, Owensboro, KY 42303 | Preschool |
| East View Elementary After School Program | 6104 Kentucky Highway 405, Owensboro, KY 42303 | Center |
| Eastview Elementary Head Start | 6104 Highway 405, Owensboro, KY 42303 | Preschool |
| Ebenezer Temple Child Development Center | 601 Center Street, Owensboro, KY 42303 | Preschool |
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| Estes Family Resource Center Child Care Program | 1675 Leitchfield Road, Owensboro, KY 42303 | Preschool |
| Helen D. Sears Family Development Center | 2225 East 18th Street, Owensboro, KY 42303 | Preschool |
| Highland After School Program | 2909 Leitchfield Road, Owensboro, KY 42303 | Center |
| K. B.'s Kid Zone | 3045 Burlew Boulevard, Owensboro, KY 42303 | Preschool |
| Kid's Town Child Care | 722 West Byers Avenue, Owensboro, KY 42303 | Preschool |
| Kiddie Academy | 315 & 323 Clay Street, Owensboro, KY 42303 | Preschool |
| Kiddie Kollege | 2724 New Hartford Road, Owensboro, KY 42303 | Preschool |
| Lea's Learning Center | 2767 Veach Road # C And # D, Owensboro, KY 42303 | Preschool |
| Leola C. Hayden Family Development Center | 1306 East 8th Street, Owensboro, KY 42303 | Preschool |
| Little Einsteins | 3940 Old Hartford Road, Owensboro, KY 42303 | Preschool |
| Little House of Learning | 409 East 25th Street, Owensboro, KY 42303 | Preschool |
| Little Ones Academy | 714 East 3rd Street, Owensboro, KY 42303 | Preschool |
| Maximilian Montessori Academy | 1041 B Spring Bank Drive, Owensboro, KY 42303 | Preschool |
| Meadow Lands After School Program | 3500 Hayden Road, Owensboro, KY 42303 | Center |
| Owensboro Catholic Elementary School, 4 - 6 Campus | 525 East 23rd Street, Owensboro, KY 42303 | Center |
| Owensboro Child Care | 300 West Byers Avenue, Owensboro, KY 42303 | Preschool |
| Owensboro Church of Christ Preschool | 3300 Highway 60 East, Owensboro, KY 42303 | Preschool |
| Settle Memorial Preschool | 201 East Fourth Street, Owensboro, KY 42303 | Preschool |
| St. Pius Tenth Day Care | 3418 Hardinsburg Road, Owensboro, KY 42303 | Preschool |
| Sweet Pea | 3271 Alvey Park Drive Suite B, Owensboro, KY 42303 | Preschool |
| Wee People Day Care | 2024 Hall Street, Owensboro, KY 42303 | Preschool |