| Oralabor KinderCare | 225 SW Oralabor Road, Ankeny, IA 50023 | Center |
| 2 For You Day Care | 6950 NE 14th St Stes 36-38, Ankeny, IA 50023 | Center |
| Ankeny Christian Academy Childcare | 1604 W 1st St, Ankeny, IA 50023 | Center |
| Ankeny Christian Childcare | 2506 SW 3rd St, Ankeny, IA 50023 | Center |
| Bright Beginnings Preschool at A First United Methodist Church | 206 SW Walnut St, Ankeny, IA 50023 | Center |
| Child's View Day Care | 118 SW State St, Ankeny, IA 50023 | Center |
| Cottontail Creek Montessori School | 7297 NW Fisher Lane, Ankeny, IA 50023 | Center |
| Creative Children Preschool | 2506 SW 3rd St, Ankeny, IA 50023 | Center |
| DMACC Child Development Center | 2006 S ANKENY BLVD BLDG 9, Ankeny, IA 50023 | Center |
| Discovery World Preschool | 914 NW Ash Dr, Ankeny, IA 50023 | Center |
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| Tender Years Daycare 1 | 110 SW Maple St, Ankeny, IA 50023 | Center |
| Tender Years Daycare 2 | 1620 SW White Birch Circle, Ankeny, IA 50023 | Center |
| The Club House Learning Center | 1932 SW Third St, Ankeny, IA 50023 | Center |
| The Tree House Growing & Learning Center | 2401 West 1st St, Ankeny, IA 50023 | Center |
| YMCA Childcare - Ashland Ridge | 2600 NW Ash Dr, Ankeny, IA 50023 | Center |
| YMCA Childcare - Crocker | 2910 SW Applewood St, Ankeny, IA 50023 | Center |
| YMCA Childcare - Northwest | 1201 W 1st, Ankeny, IA 50023 | Center |
| YMCA Childcare - Prairie Trail Elementary | 1850 SW College Ave, Ankeny, IA 50023 | Center |
| YMCA Childcare - Terrace | 310 NW School St, Ankeny, IA 50023 | Center |
| YMCA Childcare - Westwood | 2920 NW 9th St, Ankeny, IA 50023 | Center |