| Child Development Center - Johnson School | 724 W 9th St, Spencer, IA 51301 | Center |
| First English Christian Preschool | 1311 E 18th St, Spencer, IA 51301 | Center |
| Sacred Heart School Pre-Kindergarten - Spencer | 1111 4th Ave W, Spencer, IA 51301 | Center |
| Spencer Community Preschool - A Child's Garden | 724 W 9th St, Spencer, IA 51301 | Center |
| Spencer Family YMCA Dvergsten Childcare Center | 1001 11th Ave West, Spencer, IA 51301 | Center |
| Trinity Preschool & Daycare - Spencer | 900 11th Ave West, Spencer, IA 51301 | Center |
| UDMO ECP - Jefferson School | 411 E 10th St, Spencer, IA 51301 | Center |