| La Petite Academy of Iowa City | 1504 Mall Dr, Iowa City, IA 52240 | Center |
| ABC Childcare | 1 Amber Lane, Iowa City, IA 52240 | Center |
| Apple Tree Childrens Center - IAC | 325 S Dubuque St, Iowa City, IA 52240 | Center |
| Faith UCC Head Start | 1609 Deforest Ave, Iowa City, IA 52240 | Center |
| Four Oaks PAL Program | 1916 Waterfront Dr, Iowa City, IA 52240 | Center |
| Iowa City Recreation | Division / J Carroll, Iowa City, IA 52240 | Center |
| Kaleidoscope Christian Preschool | 1035 Wade St, Iowa City, IA 52240 | Center |
| Kiddie Konnection | 2707 Muscatine Ave, Iowa City, IA 52240 | Center |
| Kids Care Cooperative | 320 E College St, Iowa City, IA 52240 | Center |
| KinderFarm Preschool | 5048B Highway 6 SE, Iowa City, IA 52240 | Center |
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| Kirkwood School For Children | 1107 Kirkwood Ct, Iowa City, IA 52240 | Center |
| Longfellow Key Before & After School Program | 1130 Seymour Ave, Iowa City, IA 52240 | Center |
| NCJC - Broadway Site | 2105 Broadway St, Iowa City, IA 52240 | Center |
| NCJC - Grant Wood Preschool | 1930 Lakeside Dr, Iowa City, IA 52240 | Center |
| NCJC - Pheasant Ridge | 2651 Roberts Rd, Iowa City, IA 52240 | Center |
| NCJC- Twain Before & After School Program | 1355 Deforest Ave Rm 7, Iowa City, IA 52240 | Center |
| SHARE at Wood | 1930 Lakeside Dr, Iowa City, IA 52240 | Center |
| Stepping Stones Preschool - Iowa City | 3001 Muscatine Ave, Iowa City, IA 52240 | Center |
| Waterfront Family Resource Center | 367 Southgate Ave, Iowa City, IA 52240 | Center |