| St. Charles KinderCare | 1900 Zumbehl Rd, Saint Charles, MO 63303 | Center |
| Abc Daycare & Learning Centers Inc | # 15 Garvey Pky, Saint Charles, MO 63303 | Preschool |
| Bridgeway Child Care Center | 1570 S Main St, Saint Charles, MO 63303 | Center |
| First United Methodist Church Preschool and Mdo * | 801 First Capital Dr, Saint Charles, MO 63303 | Preschool |
| Great Beginnings Learning Center | 2183 Muegge Rd, Saint Charles, MO 63303 | Preschool |
| Hope Academy of Early Learning | 2041 Bluestone Dr, Saint Charles, MO 63303 | Preschool |
| Hope Lutheran Church Preschool | 1975 Hwy 94 South, Saint Charles, MO 63303 | Preschool |
| Just 4 Us Childcare and Learning Center | 1579 S Old Highway 94, Saint Charles, MO 63303 | Center |
| New Day Child Development Center Inc | 1530 1st Capitol Dr. South, Saint Charles, MO 63303 | Center |
| The Early Childhood Prep School | 2720 Highway 94 S Outer Road, Saint Charles, MO 63303 | Center |
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| YMCA St Charles County Branch Harris Elementary | 2800 Old Muegge Rd, Saint Charles, MO 63303 | Center |
| YMCA St Charles St Elizabeth - St Roberts Elementary School | # 1 Seton Ct, Saint Charles, MO 63303 | Center |