| A Kids Club | 313 Wood St, O Fallon, MO 63366 | Center |
| Abc Day Care and Learning Center Inc | 2796 Hwy K, O Fallon, MO 63366 | Preschool |
| Amerikids Christian Center | 1017 N Main St, O Fallon, MO 63366 | Center |
| Bee Brite Learning Center | 401 Glengate Estates Dr, O Fallon, MO 63366 | Center |
| Bella Montessori | 203 Church St, O Fallon, MO 63366 | Center |
| Carrie Kueck | 1354 Buffalo Rock Dr, O Fallon, MO 63366 | Center |
| Cornerstone Preschool | 1151 Tom Ginnever, O Fallon, MO 63366 | Preschool |
| Creative Corner Childcare Center | 1075 Highway P, O Fallon, MO 63366 | Center |
| Delmar Gardens of O Fallon Early Childhood Ed Center | 7068 S Outer 364, O Fallon, MO 63366 | Center |
| First Academy Child Care | 2688 Hwy K, O Fallon, MO 63366 | Center |
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| Holy Cross Lutheran Preschool | 8945 Veterans Memorial Pkwy, O Fallon, MO 63366 | Preschool |
| Imagination Station Preschool and Child Care | 220 Sonderen St, O Fallon, MO 63366 | Preschool |
| Just 4 Us Childcare Inc. | 1306 Feise Rd, O Fallon, MO 63366 | Center |
| Kids Academy Inc. | 7767 Highway N, O Fallon, MO 63366 | Center |
| Kids Play | 2949 Hwy K, O Fallon, MO 63366 | Preschool |
| Kids R Kids | 123 Hutchings Farm Dr, O Fallon, MO 63366 | Preschool |
| Learning Place Preschool | 8576 Veterans Memorial Pky, O Fallon, MO 63366 | Preschool |
| Little House Learning Center | 1031 Bryan Rd, O Fallon, MO 63366 | Center |
| Little Learner's Preschool O'fallon Family YMCA | 3451 Pheasant Meadows Dr, O Fallon, MO 63366 | Preschool |
| Stepping Stones Christian Academy O Fallon | 207 East Pitman, O Fallon, MO 63366 | Center |
| Tendercare Learning Center #3 | 206 Mcdonald Ln, O Fallon, MO 63366 | Preschool |
| The Learning Lodge | 140 T.r. Hughes Blvd, O Fallon, MO 63366 | Preschool |
| World of Little People | 128 E Elm St, O Fallon, MO 63366 | Center |
| YMCA St Charles Branch Mount Hope Elementary | 1099 Mount Hope, O Fallon, MO 63366 | Center |
| YMCA St Charles Co Branch Pheasant Point | 3450 Pheasant Meadows Dr, O Fallon, MO 63366 | Center |
| YMCA St Charles County - Emge Elementary | 250 O Fallon Pky, O Fallon, MO 63366 | Center |
| YMCA St Charles County Assumption School | 203 W 3rd St, O Fallon, MO 63366 | Center |
| YMCA St Charles County Branch Dardenne Elementary | 2621 Highway K, O Fallon, MO 63366 | Center |
| YMCA St Charles County Branch J L Mudd | 610 Prince Ruppert, O Fallon, MO 63366 | Center |
| YMCA St Charles County Branch Rock Creek School | 8970 Mexico Rd, O Fallon, MO 63366 | Center |
| YMCA St Charles County Branch Twin Chimney | 7396 Twin Chimneys Blvd, O Fallon, MO 63366 | Center |
| YMCA St Charles County Branch Westhoff Elementary | 900 Homefield Pky, O Fallon, MO 63366 | Center |