| Columbia KinderCare | 2416 W Ash St, Columbia, MO 65203 | Preschool |
| A Good Start | 301 W Broadway, Columbia, MO 65203 | Preschool |
| A World of Discoveries Day Care | 701 Washington Ave, Columbia, MO 65203 | Center |
| Academy of Early Childhood Learning | 3210 S Providence Rd, Columbia, MO 65203 | Center |
| Bright Start Academy | 4300 Rainbow Trout, Columbia, MO 65203 | Center |
| Carousel Playschool | 2601 W Broadway, Columbia, MO 65203 | Preschool |
| Central Missouri Community Action | 1402 W Worley St, Columbia, MO 65203 | Preschool |
| Columbia Montessori School | 3 Anderson Ave, Columbia, MO 65203 | Center |
| Country Side Nursery School | 1320 S Fairview Rd, Columbia, MO 65203 | Center |
| Curators of The University of Missouri | 909 Fairview Rd, Columbia, MO 65203 | Center |
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| Curators of The University of Missouri | 5151 S Highway 163, Columbia, MO 65203 | Center |
| Curators of The University of Missouri | 1800 W Rollins Rd, Columbia, MO 65203 | Center |
| Fairview Adventure Club | 909 Fairview Rd, Columbia, MO 65203 | Center |
| Faithwalk Ministry | 312 Ridgeway Ave, Columbia, MO 65203 | Center |
| Garden Gate School | 107 Park Hill Ave, Columbia, MO 65203 | Preschool |
| Grant Adventure Club | 10 E Broadway, Columbia, MO 65203 | Center |
| Green Meadows Preschool | 1000 Green Meadows Rd, Columbia, MO 65203 | Preschool |
| Loving With Logic Playschool | 3908 Keystone Ct, Columbia, MO 65203 | Center |
| Mothers Morning Out | 3200 Chapel Hill Rd, Columbia, MO 65203 | Preschool |
| Patrick Enterprises | 404 Mcbaine Ave, Columbia, MO 65203 | Center |
| Ridgeway Adventure Club | 107 E Sexton Rd, Columbia, MO 65203 | Center |
| Rock Bridge Adventure Club | 5151 S Highway 163, Columbia, MO 65203 | Center |
| Rock Bridge Preschool | 102 Seivelle Ave, Columbia, MO 65203 | Preschool |
| Russell Adventure Club | 1800 W Rollins Rd, Columbia, MO 65203 | Center |
| Southwest Play School | 1600 W Broadway, Columbia, MO 65203 | Preschool |
| The Apple School | 5155 Providence Rd S, Columbia, MO 65203 | Center |
| The Curators of The University of Missouri | 201 Park De Ville Dr, Columbia, MO 65203 | Center |
| The Curators of The University of Missouri | 2200 Nifong Blvd W, Columbia, MO 65203 | Center |
| Tiger Tots Academy | 606 Claudell Ln, Columbia, MO 65203 | Preschool |
| Trinity Lutheran Church of Columbia Missouri | 2201 W Rollins Rd, Columbia, MO 65203 | Center |
| Turn The Page Child Development Center | 1201 Jewell Ave, Columbia, MO 65203 | Center |
| United Cerebal Palsy Heartland Child Development Center | 3804 Santiago Dr, Columbia, MO 65203 | Center |
| University of Missouri Student Parent Center | 602 University Village, Columbia, MO 65203 | Preschool |
| West Blvd Adventure Club | 319 W Boulevard North, Columbia, MO 65203 | Center |