| Blocks For Tots Preschool | 1301 E. TAYLOR, Harlingen, TX 78550 | Preschool |
| Bonita Park Head Start CDC | 601 S Rangerville R Apt 29-30, Harlingen, TX 78550 | Preschool |
| Bright Minds Academy | 1201 E VAN BUREN AVE, Harlingen, TX 78550 | Center |
| Calvary Christian Day Care Center | 1815 N 7th St, Harlingen, TX 78550 | Preschool |
| Casa Grande Head Start Child Development Center | 106 Troywood Circle 104 1/2 Troywood Circle, Harlingen, TX 78550 | Preschool |
| Centro De Fe Day Care | 2010 LaSalle, Harlingen, TX 78550 | Preschool |
| Child Care Center of Harlingen | 104 W Taylor, Harlingen, TX 78550 | Preschool |
| For Kids Only | 1201 E Van Buren, Harlingen, TX 78550 | Preschool |
| Grace Preschool & Childcare Center | 2220 Haine Dr. Suite 47, Harlingen, TX 78550 | Preschool |
| Great Beginnings Preschool and Child Development Center | 602 Morgan Blvd, Harlingen, TX 78550 | Preschool |
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| Harlingen Child Development Center | 701 E Madison, Harlingen, TX 78550 | Preschool |
| Harlingen Head Start Child Dev Center | 829 Filmore Street, Harlingen, TX 78550 | Preschool |
| Jellybeans Educational Daycare Center | 2208 Camelot Plaza Circle, Harlingen, TX 78550 | Preschool |
| Journey Preschool - A Ministry of FUMC | 321 E Harrison, Harlingen, TX 78550 | Preschool |
| Kids Carrousel Day Care | 625 Markowsky, Harlingen, TX 78550 | Preschool |
| Kids Day Care | 301 Rangerville Rd., Harlingen, TX 78550 | Preschool |
| Kids Playhouse | 1318 Haverford, Harlingen, TX 78550 | Preschool |
| Le Moyne Gardens Headstart | 3221 North 25th St., Harlingen, TX 78550 | Preschool |
| Little Bloomers | 1017 S. F Street, Harlingen, TX 78550 | Preschool |
| Little Discoveries Learning Center | 21048 Montezuma Rd. 7-a Montezuma, Harlingen, TX 78550 | Preschool |
| Little Lou's A.B.C. Day Care Center | 1813 Bell St., Harlingen, TX 78550 | Preschool |
| Little Treasures North Child Development Center | 1501 North 28th Street Suite C & D, Harlingen, TX 78550 | Preschool |
| Los Vecinos | 702 South M St Apt 309 & 310, Harlingen, TX 78550 | Preschool |
| Magic Kingdom Learning Center | 1422 Ball St., Harlingen, TX 78550 | Preschool |
| Magic Kingdom Learning Center 2 | 1820 Morgan Blvd., Harlingen, TX 78550 | Preschool |
| Montessori Academy | 3943 Bourbon, Harlingen, TX 78550 | Preschool |
| Nazarene Child Care Center | 2001 North 7th St, Harlingen, TX 78550 | Preschool |
| Play Time Planet Center | 1510 S. Expressway 83, Harlingen, TX 78550 | Center |
| Play and Learn Day Care Center | 1701 East Van Buren, Harlingen, TX 78550 | Preschool |
| Shooting Star Learning Center | 1646 Throckmorton, Harlingen, TX 78550 | Preschool |
| St. Albans Day Care Center | 1417 Austin, Harlingen, TX 78550 | Center |
| TSTC Child Development Headstart | 2702 Dogwood, Harlingen, TX 78550 | Preschool |
| TSTC Early Head Start Child Development | 2225 North 28th St, Harlingen, TX 78550 | Preschool |
| The Garden | 2809 N 7th St, Harlingen, TX 78550 | Preschool |
| The Little Red School House Child Development Center | 1502 Dixieland Road, Harlingen, TX 78550 | Preschool |
| Travis Head Start Child Development Center | 600 E. Polk Street, Harlingen, TX 78550 | Preschool |
| Valley Learning Center for Kids | 1916 E. Harrison Street, Harlingen, TX 78550 | Preschool |
| Wesley Preparatory School | 1406 E Austin, Harlingen, TX 78550 | Preschool |
| Zavala Child Development Head Start Center | 421 Lafayette St, Harlingen, TX 78550 | Preschool |