| Bethany Christian School | 200 W Bethany Ct, Thousand Oaks, CA 91360 | Preschool |
| CVUSD's NFL at Park Oaks | 1335 Calle Bouganvilla, Thousand Oaks, CA 91360 | Center |
| California Lutheran University Preschool | 3950 Mountclef Dr, Thousand Oaks, CA 91360 | Preschool |
| Children's Learning Center | 2993 Crescent Way, Thousand Oaks, CA 91360 | Center |
| Christian Preschool | 301 W Avenida De Las Flores, Thousand Oaks, CA 91360 | Preschool |
| First Baptist Church Day Care and Preschool | 1250 Erbes Road, Thousand Oaks, CA 91360 | Preschool |
| Hirest Nursery School | 384 Erbes Road, Thousand Oaks, CA 91360 | Preschool |
| Honey Tree Nursery School | 1 West Avenida De Los Arboles, Thousand Oaks, CA 91360 | Preschool |
| Montessori Children's Place | 390 Arcturus St., Thousand Oaks, CA 91360 | Preschool |
| Notre Dame Learning Center | 1776 Hendrix Ave., Thousand Oaks, CA 91360 | Preschool |
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| Pacific Camps Thousand Oaks | 588 Camino Manzanas, Thousand Oaks, CA 91360 | Center |
| Pinecrest School | 449 Wilbur Road, Thousand Oaks, CA 91360 | Preschool |
| Temple Adat Elohim Preschool | 2420 E. Hirest Drive, Thousand Oaks, CA 91360 | Preschool |
| Temple Etz Chaim Preschool | 1080 Janss Road, Thousand Oaks, CA 91360 | Preschool |
| Thousand Oaks Head Start | 243 E Wilbur Apt 103&106, Thousand Oaks, CA 91360 | Preschool |