| La Petite Academy of Brandon | 606 E. Windhorst Road, Brandon, FL 33510 | Preschool |
| La Petite Academy of Valrico | 1011 Greenhollow Lane, Valrico, FL 33596 | Center |
| Seffner KinderCare | 2524 S Parsons Ave, Seffner, FL 33584 | Preschool |
| Walden Lake KinderCare | 1701 W Timberlane Dr, Plant City, FL 33566 | Preschool |
| A + Learning Center | 221 W. Alexander Street, Plant City, FL 33563 | Preschool |
| A Child's Haven Private Preschool | 1520 Brentwood Hills Boulevard, Valrico, FL 33594 | Preschool |
| A Children's Kastle Early Learning Center | 950 S. Mulrennan Road, Valrico, FL 33594 | Preschool |
| Above and Beyond Childcare | 9472 Mcintosh Road, Dover, FL 33527 | Center |
| Apostles Lutheran Church Preschool | 200 N. Kingsway Road, Brandon, FL 33510 | Preschool |
| Autumn Leaf Academy | 3007 Daniels Road, Plant City, FL 33566 | Preschool |
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| B & G Day Care and Kindergarten | 14419 Dr Mlk Boulevard, Dover, FL 33527 | Preschool |
| Bealsville Head Start Center | 5100 Horton Road, Plant City, FL 33567 | Preschool |
| Bobbie's Busy Bee | 4105 Glen Road, Lakeland, FL 33810 | Preschool |
| Boys & Girls Club Dover | 2820 South Gallagher Road, Dover, FL 33527 | Center |
| Brandon Sports and Aquatic Center | 405 Beverly Blvd, Brandon, FL 33511 | Preschool |
| Bright Beginnings | 2103 Mud Lake Road, Plant City, FL 33566 | Center |
| Bright Imaginations | 6075 South Florida Ave., Lakeland, FL 33813 | Preschool |
| Brighter Kids | 2207 Durant Road, Valrico, FL 33596 | Center |
| Central Baptist Christian School | 402 E. Windhorst Road, Brandon, FL 33510 | Center |
| Children's Life Academy | 3120 S. Miller Road, Valrico, FL 33596 | Center |
| Children's Nest Day School | 1311 Mendonsa Road, Plant City, FL 33563 | Preschool |
| Cky Learning Center | 6314 1/2 Sweetwater Drive W, Lakeland, FL 33811 | Preschool |
| Colors R Us Child Development Center | 1512 George Jenkins Blvd, Lakeland, FL 33815 | Center |
| Country Kids | 3802 N. Gallagher Road, Plant City, FL 33565 | Center |
| Crossroads Baptist Church | 10405 S. Highway 39, Lithia, FL 33547 | Center |
| Discovery Point #54 | 3472 Kings Commercial Ct, Valrico, FL 33594 | Preschool |
| Early Childhood Learning Center Airside | 3135 Airside Center Drive, Lakeland, FL 33811 | Preschool |
| Easter Seals Child Development at Teen Parent East | 1202 Grant Street, Plant City, FL 33563 | Center |
| First Baptist Learning Center | 402 N. Collins Street, Plant City, FL 33566 | Center |
| First Presbyterian Learning Center | 404 W. Reynolds Street, Plant City, FL 33563 | Center |
| Foundation Christian Academy | 3955 Lithia Pinecrest Road, Valrico, FL 33596 | Preschool |
| Fun Factory Preschool | 410 S. Collins Street, Plant City, FL 33563 | Preschool |
| Golden Rule Child Care Centers | 104 Golden Rule Lane, Plant City, FL 33566 | Center |
| Golden Rule Child Care Centers | 105 Thrasher Road, Plant City, FL 33566 | Center |
| Gospel Tabernacle Learning Center | 801 Wilkins Avenue, Plant City, FL 33563 | Center |
| HCPS - Dover Elementary Headstart Vpk | 3035 Nelson Ave., Dover, FL 33527 | Preschool |
| HCPS - Lincoln Elementary Headstart Vpk | 1207 E. Renfro St., Plant City, FL 33566 | Preschool |
| HCPS - Trapnell Elementary Headstart Vpk | 1605 West Trapnell Rd, Plant City, FL 33567 | Preschool |
| Happy Day Preschool | 722 S. Lithia Pinecrest Road, Brandon, FL 33511 | Preschool |
| Hazel Perkins Gray Child Development Center | 1620 W Parker St., Lakeland, FL 33815 | Center |
| Horizon Early Learning Preschool | 1720 S. Saint Cloud Avenue, Valrico, FL 33594 | Preschool |
| Jay Care Enrichment Academy | 4609 Horton Road, Plant City, FL 33567 | Center |
| Joyful Hearts Preschool a Ministry of Lakela | 1725 Parker Road, Lakeland, FL 33811 | Preschool |
| Kathleen Preschool | 1100 Red Devil Way #c7, Lakeland, FL 33815 | Center |
| Kathleen Preschool Academy Preschool | 2600 Crutchfield Road #c8, Lakeland, FL 33805 | Preschool |
| Kids Club Early Learning Center | 1108 Goldfinch Drive, Plant City, FL 33563 | Center |
| Kids R Kids #8 FL | 4321 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| Kids R Tops | 215 N. Kingsway Road, Brandon, FL 33510 | Center |
| Kids World Enrichment Center II | 932 Martin Luther King Junior Bou ..., Lakeland, FL 33815 | Center |
| Kimberly's Infant Care Center | 605 S. Drane Street, Plant City, FL 33563 | Preschool |
| Kinder Kountry | 12660 Sydney Road, Dover, FL 33527 | Center |
| Learn & Grow Pre-school | 2501 Durant Road, Valrico, FL 33596 | Preschool |
| Linda's Little People | 1406 N. Wheeler Street, Plant City, FL 33563 | Center |
| Little Believers Daycare and Learning Center | 1700 George Jenkins Blvd, Lakeland, FL 33815 | Preschool |
| Little Explorer's Learning Academy | 301 E. Alsobrook Street, Plant City, FL 33563 | Center |
| Little Footsteps of Lakeland | 6595 South Florida Avenue, Lakeland, FL 33813 | Preschool |
| Little Munchkins | 702 S. Ever Street, Plant City, FL 33563 | Center |
| Little World at Shepherd Road | 1333 Shepherd Rd, Lakeland, FL 33811 | Preschool |
| Nativity Catholic School | 705 E. Brandon Boulevard, Brandon, FL 33511 | Center |
| New Jerusalem Christian Academy | 3101 Kingsway Road, Seffner, FL 33584 | Preschool |
| Pathway of Lakeland | 1942 West Memorial Blvd, Lakeland, FL 33815 | Center |
| Plant City Head Start Center | 1304 Alsobrook Street, Plant City, FL 33563 | Preschool |
| Plant City High Little School | 1 Raider Place, Plant City, FL 33563 | Preschool |
| Plant City Learning Center | 651 E. Alexander Street, Plant City, FL 33563 | Center |
| Publix Child Development Center | 345 N Galloway Rd, Lakeland, FL 33815 | Center |
| RCMA Central Plant City Child Development Center | 508 Lakewood Avenue, Plant City, FL 33563 | Preschool |
| RCMA Dover Child Development Center | 3238 San Jose Mission Drive, Dover, FL 33527 | Preschool |
| RCMA Madonia Child Development Center | 4438 Academy Dr, Mulberry, FL 33860 | Preschool |
| RCMA Mulberry Child Development Center | 4441 Academy Drive, Mulberry, FL 33860 | Center |
| RCMA Plant City Child Development Center | 1402 W. Knights Griffin Road, Plant City, FL 33565 | Preschool |
| RCMA Sam Allen Child Development Center | 2701 N. Demont Mollin Road, Plant City, FL 33565 | Preschool |
| RCMA Westside Village Child Development Center | 3102 Sammonds Road, Plant City, FL 33563 | Preschool |
| Shepherd Road Christian Academy | 1217 Shepherd Road, Lakeland, FL 33811 | Center |
| Shiloh Baptist Church Preschool | 905 W. Terrace Street, Plant City, FL 33563 | Center |
| Shining Stars Preschool Academy | 603 E. Morgan Street, Brandon, FL 33510 | Preschool |
| Smart Kids Academy | 3512 W. Baker Street, Plant City, FL 33563 | Preschool |
| Smart Start Youth & Families Services | 2727 Shepherd Rd, Lakeland, FL 33811 | Center |
| Smart Start Youth & Family Services | 5500 Yates Road, Lakeland, FL 33811 | Center |
| Springhead Baptist Learning Center | 3106 S. Wiggins Road, Plant City, FL 33566 | Center |
| St. Clement Early Childhood Center | 1104 N. Alexander Street, Plant City, FL 33563 | Preschool |
| St. Paul Lutheran School | 4450 Harden Boulevard, Lakeland, FL 33813 | Center |
| Strawberry Hills Head Start & Early Head Start | 701 Tillman Place, Plant City, FL 33566 | Preschool |
| Strawberry Patch | 2805 W. Reynolds Street, Plant City, FL 33563 | Center |
| Taylor Learning Center | 827 White Lane, Lakeland, FL 33813 | Preschool |
| The Brandon Montessori School at River Hills | 4223 Lynx Paw Trail, Valrico, FL 33596 | Preschool |
| The Learning Train Academy | 736 W. Dr Mlk Blvd, Seffner, FL 33584 | Center |
| The Word Of His Grace L.D.C. Preschool | 2506 S. Parsons Avenue, Seffner, FL 33584 | Preschool |
| Tots Active Learning Center | 121 W. Prosser Drive, Plant City, FL 33563 | Center |
| Valrico Child Care | 1405 S. St. Cloud Avenue, Valrico, FL 33594 | Center |
| Walden Lake Early Learning Center | 2730 Turkey Creek Road, Plant City, FL 33566 | Preschool |
| Wee Care Learning Academy | 219 Skywood Dr, Valrico, FL 33594 | Center |
| YMCA - Winston Kid Care | 3415 Swindell Road, Lakeland, FL 33810 | Center |
| Younger Years Learning Center | 1905 Lenna Avenue, Seffner, FL 33584 | Center |