| A Learning Center of Hope | 25999 Old 41 Road, Bonita Springs, FL 34135 | Preschool |
| After School Programs at Seagate | 650 Seagate Dr, Naples, FL 34103 | Center |
| Allie Gator Playskool | 4125 Pine Road, Fort Myers, FL 33908 | Center |
| Bear's Den Childcare Center | 1665 Medical Blvd Suite B, Naples, FL 34110 | Preschool |
| Bobbie Noonan's Child Care at Bonita Springs | 28052 Mango Drive, Bonita Springs, FL 34134 | Preschool |
| Bonita Springs Elementary Preschool | 10701 Dean Street, Bonita Springs, FL 34135 | Preschool |
| Cedar Montessori School North | 10904 Winterview Drive, Naples, FL 34109 | Preschool |
| Cheers Early Learning Center II | 19660 S Tamiami Trail, Fort Myers, FL 33908 | Preschool |
| Children's Montessori School | 2535 Northbrooke Plaza Drive, Naples, FL 34119 | Preschool |
| Creation Station Wee Christian Learning Center | 8000 Goodlette Road N, Naples, FL 34109 | Preschool |
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| Creative World School Three Oaks | 19671 Cypress View Drive, Fort Myers, FL 33967 | Preschool |
| Destiny Learning Center | 7690 Running Buck Court, Naples, FL 34120 | Center |
| Eagle's Nest Christian Academy | 6920 Immokalee Road, Naples, FL 34119 | Preschool |
| Educare Academy and Child Care Centers | 10220 W Terry St, Bonita Springs, FL 34135 | Preschool |
| Family Resource Center at Florida Gulf Coast | 10501 Fgcu Boulevard South, Fort Myers, FL 33965 | Preschool |
| First Baptist Academy | 3000 Orange Blossom Drive, Naples, FL 34109 | Center |
| Gospel Baptist Church & Christian School | 24861 Old 41 Road, Bonita Springs, FL 34135 | Center |
| Grace Community School of Bonita Springs | 8971 Brighton Lane, Bonita Springs, FL 34135 | Preschool |
| Grace Community School of Naples Park | 871 100th Avenue North, Naples, FL 34108 | Preschool |
| Little Peoples School | 868 94th Avenue N, Naples, FL 34108 | Preschool |
| Living Waters Academy | 27911 Vermont Street, Bonita Springs, FL 34135 | Center |
| Montessori Academy of Naples | 2659 Professional Circle Suite 1118, Naples, FL 34119 | Preschool |
| Mrs Hamrick's Preschool Learning Center | 3971 Via Del Rey, Bonita Springs, FL 34134 | Center |
| Naples Park Elementary Preschool | 685 - 111th Avenue North, Naples, FL 34108 | Preschool |
| Noah's Ark Academy | 860 102nd Avenue N, Naples, FL 34108 | Preschool |
| Noah's Ark Academy Bonita Springs | 27970 Crown Lake Blvd #5, Bonita Springs, FL 34135 | Center |
| Osceola Elementary Preschool | 5470 Osceola Trail, Naples, FL 34109 | Preschool |
| Our Savior Preschool and Child Care Center | 1955 Curling Avenue, Naples, FL 34109 | Preschool |
| Peppermint Tree Preschool and Childcare Center | 17567 Liberty Square Circle, Fort Myers, FL 33967 | Preschool |
| Pinewoods Elementary Preschool | 11900 Stoneybrook Golf Drive, Estero, FL 33928 | Preschool |
| Precious Cargo Academy | 5200 Crayton Road, Naples, FL 34103 | Preschool |
| Preschool at The Lamb | 19691 Cypress View Drive, Fort Myers, FL 33967 | Preschool |
| RCMA Bonita Child Development Center | 13190 Growers Road, Bonita Springs, FL 34135 | Center |
| Royal Palm Academy | 16100 Livingston Rd, Naples, FL 34110 | Preschool |
| Saint John the Evangelist Early Learning & Child Care | 625 111th Avenue N, Naples, FL 34108 | Center |
| San Carlos Child Care | 19133 Acorn Road Se, Fort Myers, FL 33967 | Preschool |
| Shalom Preschool | 4630 Pine Ridge Road, Naples, FL 34119 | Preschool |
| Small World Early Learning Center | 727 108th Avenue, Naples, FL 34108 | Preschool |
| Sports CLUB at Bonita Recreational Center | 26740 Pine Ave, Bonita Springs, FL 34135 | Center |
| Sports CLUB at Laurel Oaks | 7800 Immokalee Road, Naples, FL 34119 | Center |
| Sports CLUB at Naples Park | 685 111th Avenue N, Naples, FL 34108 | Center |
| Sports CLUB at Osceola | 5770 Osceola Trail, Naples, FL 34109 | Center |
| Sports CLUB at Pelican Marsh | 9480 Airport Road N, Naples, FL 34109 | Center |
| Sports CLUB at Veterans Memorial | 15960 Veterans Memorial Blvd, Naples, FL 34110 | Center |
| Sports CLUB at Vineyards | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| The Creation Station Wee Christian Learning Center | 8000 Goodlette Rd N, Naples, FL 34109 | Preschool |
| The Learning Tree of Naples | 875 108th Ave N, Naples, FL 34108 | Preschool |
| The Village School | 6000 Goodlette Frank Road N, Naples, FL 34108 | Preschool |
| Vanderbilt Presbyterian Childcare & Learning Center | 1225 Piper Blvd, Naples, FL 34110 | Preschool |
| Veterans Community Park | 1895 Veteran Park Drive, Naples, FL 34109 | Center |
| Vineyards Community Park | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| Vineyards Elementary Preschool | 6225 Arbor Boulevard West, Naples, FL 34119 | Preschool |
| Waves of Wonder | 7740 Preserve Lane 2, Naples, FL 34119 | Preschool |
| Wonder Years Preschool | 9751 Bonita Beach Road, Bonita Springs, FL 34135 | Preschool |
| YMCA Child Development Center | 5450 Ymca Road, Naples, FL 34109 | Preschool |
| YMCA Early Learning Center at Veterans | 15960 Veterans Memorial Blvd, Naples, FL 34110 | Preschool |
| YMCA of the Palms Gaynor Child Development | 2510 Museum Drive, Naples, FL 34109 | Preschool |