| 123 Grow With Me Infant Toddler Center | 2727 Bayshore Drive, Naples, FL 34112 | Center |
| 123 Grow With Me Learning Center | 2737 Bayshore Drive, Naples, FL 34112 | Preschool |
| A Learning Center of Hope | 25999 Old 41 Road, Bonita Springs, FL 34135 | Preschool |
| A Step Up at Golden Gate | 2925 Magnolia Pond Drive, Naples, FL 34116 | Center |
| ABC Academy Learning Center | 5043 Coronado Parkway, Naples, FL 34116 | Preschool |
| After School Programs South at Calusa | 4600 Santa Barbara Blvd, Naples, FL 34104 | Center |
| After School Programs at Big Cypress | 3250 Golden Gate Blvd W, Naples, FL 34120 | Center |
| After School Programs at Golden Terrace | 2711 44th Terrace Sw, Naples, FL 34116 | Center |
| After School Programs at Lake Park | 1295 14th Avenue N, Naples, FL 34102 | Center |
| After School Programs at Mike Davis | 3215 Magnolia Pond Rd, Naples, FL 34116 | Center |
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| After School Programs at Seagate | 650 Seagate Dr, Naples, FL 34103 | Center |
| Ana's Little Angels Inclusion Learning Center | 2400 Kirkwood Avenue, Naples, FL 34112 | Preschool |
| Bear's Den Childcare Center | 1665 Medical Blvd Suite B, Naples, FL 34110 | Preschool |
| Bear's Den Preschool-Downtown Naples Hospital | 350 7th Street N, Naples, FL 34102 | Preschool |
| Bobbie Noonan's Child Care at Bonita Springs | 28052 Mango Drive, Bonita Springs, FL 34134 | Preschool |
| Bonita Springs Elementary Preschool | 10701 Dean Street, Bonita Springs, FL 34135 | Preschool |
| Bottles Blocks and Books | 2772 Santa Barbara Blvd, Naples, FL 34116 | Preschool |
| Cedar Montessori School North | 10904 Winterview Drive, Naples, FL 34109 | Preschool |
| Child's Path Preschool | 3144 Santa Barbara Blvd, Naples, FL 34116 | Preschool |
| Children's Montessori School | 2535 Northbrooke Plaza Drive, Naples, FL 34119 | Preschool |
| Creation Station Wee Christian Learning Center | 8000 Goodlette Road N, Naples, FL 34109 | Preschool |
| Creative Corner Preschool | 1397 13th Avenue N, Naples, FL 34102 | Center |
| Destiny Learning Center | 7690 Running Buck Court, Naples, FL 34120 | Center |
| Eagle's Nest Christian Academy | 6920 Immokalee Road, Naples, FL 34119 | Preschool |
| East Naples United Methodist Church Learning Center | 2701 Airport Road S, Naples, FL 34112 | Preschool |
| Educare Academy and Child Care Centers | 10220 W Terry St, Bonita Springs, FL 34135 | Preschool |
| First Baptist Academy | 3000 Orange Blossom Drive, Naples, FL 34109 | Center |
| First Presbyterian Preschool | 250 6th Street S, Naples, FL 34102 | Preschool |
| Fleischmann Park Community Center | 1600 Fleischmann Boulevard, Naples, FL 34102 | Center |
| Fun Time Early Childhood Academy | 102 12th Street North, Naples, FL 34102 | Preschool |
| Golden Gate Community Center | 4701 Golden Gate Parkway, Naples, FL 34116 | Preschool |
| Golden Gate Elementary Preschool | 4911 20th Place Southwest, Naples, FL 34116 | Preschool |
| Golden Gate High School Little Titans | 2925 Magnolia Pond Drive, Naples, FL 34116 | Preschool |
| Golden Terrace Elementary Preschool | 2711 44th Terrace Southwest, Naples, FL 34116 | Preschool |
| Gospel Baptist Church & Christian School | 24861 Old 41 Road, Bonita Springs, FL 34135 | Center |
| Grace Community School of Bonita Springs | 8971 Brighton Lane, Bonita Springs, FL 34135 | Preschool |
| Grace Community School of Golden Gate | 5524 19th Court Sw, Naples, FL 34116 | Preschool |
| Grace Community School of Naples Park | 871 100th Avenue North, Naples, FL 34108 | Preschool |
| Grace Lutheran Preschool | 860 Banyon Blvd, Naples, FL 34102 | Preschool |
| Here We Grow Child Development Center | 5269 Golden Gate Parkway, Naples, FL 34116 | Preschool |
| Kiddie Korral On The Parkway | 3060 62nd Street Sw, Naples, FL 34116 | Preschool |
| LWIT Learning Partners Early Education | 3702 Estey Ave, Naples, FL 34104 | Center |
| Lambs of Faith Preschool | 4150 Goodlette Road N, Naples, FL 34103 | Preschool |
| Little Ducks Day School | 4025 10th Street North Unit 1&2, Naples, FL 34103 | Preschool |
| Little Peoples School | 868 94th Avenue N, Naples, FL 34108 | Preschool |
| Living Waters Academy | 27911 Vermont Street, Bonita Springs, FL 34135 | Center |
| Louise Hasse Community Center | 3390 Golden Gate Blvd W, Naples, FL 34120 | Preschool |
| Methodist Day School | 388 First Ave South, Naples, FL 34102 | Preschool |
| Mike Davis Elementary Preschool | 3215 Magnolia Dr, Naples, FL 34116 | Preschool |
| Montessori Academy of Naples | 2659 Professional Circle Suite 1118, Naples, FL 34119 | Preschool |
| Moorings Presbyterian Preschool | 791 Harbour Drive, Naples, FL 34103 | Preschool |
| Mrs Hamrick's Preschool Learning Center | 3971 Via Del Rey, Bonita Springs, FL 34134 | Center |
| Naples Adventist Christian School | 2629 Horseshoe Dr, Naples, FL 34104 | Preschool |
| Naples Christian Academy Preschool | 3161 Santa Barbara Boulevard, Naples, FL 34116 | Center |
| Naples Park Elementary Preschool | 685 - 111th Avenue North, Naples, FL 34108 | Preschool |
| Naples Preschool Academy | 1275 Airport Road S, Naples, FL 34104 | Preschool |
| Nicaea Academy | 2200 Santa Barbara Blvd, Naples, FL 34116 | Center |
| Noah's Ark Academy | 860 102nd Avenue N, Naples, FL 34108 | Preschool |
| Noah's Ark Academy Bonita Springs | 27970 Crown Lake Blvd #5, Bonita Springs, FL 34135 | Center |
| Osceola Elementary Preschool | 5470 Osceola Trail, Naples, FL 34109 | Preschool |
| Our Savior Preschool and Child Care Center | 1955 Curling Avenue, Naples, FL 34109 | Preschool |
| Precious Cargo Academy | 5200 Crayton Road, Naples, FL 34103 | Preschool |
| RCMA Bonita Child Development Center | 13190 Growers Road, Bonita Springs, FL 34135 | Center |
| Royal Palm Academy | 16100 Livingston Rd, Naples, FL 34110 | Preschool |
| Saint Ann Catholic School | 542 8th Ave S, Naples, FL 34102 | Preschool |
| Saint John the Evangelist Early Learning & Child Care | 625 111th Avenue N, Naples, FL 34108 | Center |
| Sandcastle Day Care | 100 Cape Sable Drive, Naples, FL 34104 | Preschool |
| Sandcastle Day Care | 6028 Radio Road #14, Naples, FL 34104 | Preschool |
| Shadowlawn Elementary Preschool | 2161 Shadowlawn Drive, Naples, FL 34112 | Preschool |
| Shalom Preschool | 4630 Pine Ridge Road, Naples, FL 34119 | Preschool |
| Small World Early Learning Center | 727 108th Avenue, Naples, FL 34108 | Preschool |
| So Big So Bright | 2960 South Horseshoe Drive #1000, Naples, FL 34104 | Preschool |
| Sports CLUB at Bonita Recreational Center | 26740 Pine Ave, Bonita Springs, FL 34135 | Center |
| Sports CLUB at Golden Gate | 4911 20th Place Sw, Naples, FL 34116 | Center |
| Sports CLUB at Laurel Oaks | 7800 Immokalee Road, Naples, FL 34119 | Center |
| Sports CLUB at Naples Park | 685 111th Avenue N, Naples, FL 34108 | Center |
| Sports CLUB at Osceola | 5770 Osceola Trail, Naples, FL 34109 | Center |
| Sports CLUB at Pelican Marsh | 9480 Airport Road N, Naples, FL 34109 | Center |
| Sports CLUB at Poinciana Elementary | 2825 Airport Road S, Naples, FL 34105 | Center |
| Sports CLUB at Shadowlawn | 2161 Shadowlawn Drive, Naples, FL 34112 | Center |
| Sports CLUB at Veterans Memorial | 15960 Veterans Memorial Blvd, Naples, FL 34110 | Center |
| Sports CLUB at Vineyards | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| St. Elizabeth Seton | 2760 52nd Terrace Sw, Naples, FL 34116 | Preschool |
| TOPS Academy | 1371 Shadowlawn Drive, Naples, FL 34104 | Preschool |
| The Children's House Learning Center | 2310 Hunter Blvd, Naples, FL 34116 | Preschool |
| The Creation Station Wee Christian Learning Center | 8000 Goodlette Rd N, Naples, FL 34109 | Preschool |
| The Learning Tree of Naples | 875 108th Ave N, Naples, FL 34108 | Preschool |
| The Salvation Army | 3180 Estey Avenue, Naples, FL 34104 | Center |
| The Village School | 6000 Goodlette Frank Road N, Naples, FL 34108 | Preschool |
| Vanderbilt Presbyterian Childcare & Learning Center | 1225 Piper Blvd, Naples, FL 34110 | Preschool |
| Veterans Community Park | 1895 Veteran Park Drive, Naples, FL 34109 | Center |
| Vineyards Community Park | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| Vineyards Elementary Preschool | 6225 Arbor Boulevard West, Naples, FL 34119 | Preschool |
| Waves of Wonder | 7740 Preserve Lane 2, Naples, FL 34119 | Preschool |
| Windmill Creek Academy | 2682 South Horseshoe Court, Naples, FL 34104 | Preschool |
| Wonder Years Preschool | 9751 Bonita Beach Road, Bonita Springs, FL 34135 | Preschool |
| YMCA Child Development Center | 5450 Ymca Road, Naples, FL 34109 | Preschool |
| YMCA Early Learning Center at Veterans | 15960 Veterans Memorial Blvd, Naples, FL 34110 | Preschool |
| YMCA of the Palms Gaynor Child Development | 2510 Museum Drive, Naples, FL 34109 | Preschool |