| Children's House Montessori | 15711 Monastery Rd, Peosta, IA 52068 | Center |
| Dubuque Childcare Center | 3100 Cedar Crest Ridge Ste 5, Dubuque, IA 52003 | Center |
| Dubuque Community Y Creative Learning Center | 35 N Booth St, Dubuque, IA 52001 | Center |
| Dubuque Montessori School | 2605 Pennsylvania Ave, Dubuque, IA 52001 | Center |
| Early Learners CDC on Duggan | 610 Duggan Dr, Dubuque, IA 52003 | Center |
| Early Learners CDC on Windsor | 3495 Windsor Ave, Dubuque, IA 52001 | Center |
| East Dubuque Elementary School Preschool | 100 N School Rd, East Dubuque, IL 61025 | Preschool |
| Finley - DCY Childcare | 300 N Grandview Ave, Dubuque, IA 52001 | Center |
| Frog Hollow Kid Campus | 5005 Asbury Rd, Dubuque, IA 52002 | Center |
| Grandview Child Development Center | 3342 John Wesley Dr, Dubuque, IA 52002 | Center |
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| Hills And Dales Childcare Center | 3505 Stoneman Rd, Dubuque, IA 52001 | Center |
| Holy Ghost Early Childhood | 2981 Central Ave, Dubuque, IA 52001 | Center |
| Key West Early Childhood Center | 10244 Key West Dr, Dubuque, IA 52003 | Center |
| Kids Of The Kingdom Child Care - Dubuque Comm Y | 2899 Hales Mill Rd, Dubuque, IA 52002 | Center |
| Little Rascals Preschool and Daycare | 1125 Rockdale Rd, Dubuque, IA 52003 | Center |
| Little Treasures Preschool - Daycare | 2035 Radford Rd, Dubuque, IA 52002 | Center |
| Mercy Child Development Center & Preschool | 250 Mercy Dr, Dubuque, IA 52001 | Center |
| Mini-Masterpieces Preschool | 1205 Cleveland Ave, Dubuque, IA 52003 | Center |
| NICC Child Development Center - Peosta | 10250 Sundown Rd, Peosta, IA 52068 | Center |
| Noah's Ark Preschool - Dubuque | 1755 Delhi St, Dubuque, IA 52001 | Center |
| ONV - Bluff Street Head Start | 1499 Bluff St, Dubuque, IA 52001 | Center |
| ONV - Prescott Head Start | 1151 White St, Dubuque, IA 52001 | Center |
| ONV - St John Head Start | 1458 Locust St, Dubuque, IA 52001 | Center |
| ONV - St Mark Head Start | 1201 Locust St, Dubuque, IA 52001 | Center |
| ONV - West Locust Head Start | 715 W Locust, Dubuque, IA 52001 | Center |
| ONV - Westminster Head Start | 2155 University Ave, Dubuque, IA 52001 | Center |
| Our Redeemer Lutheran Preschool | 2145 John F Kennedy Road, Dubuque, IA 52002 | Center |
| Pooh's Corner Children's Center | 3507 Keystone Dr, Dubuque, IA 52001 | Center |
| Prodigy Child Development | 715 W Locust St, Dubuque, IA 52001 | Center |
| Resurrection Early Childhood Center | 4320 Asbury Rd, Dubuque, IA 52002 | Center |
| Romper Stompers Childcare Center | 2307 Central Avenue, Dubuque, IA 52001 | Center |
| Seton Catholic School - Peosta | 7495 Burds Road, Peosta, IA 52068 | Center |
| St Anthony Early Childhood and OLG | 2160 St Ambrose St, Dubuque, IA 52001 | Center |
| St Columbkille Early Childhood | 1220 Rush St, Dubuque, IA 52001 | Center |
| St Joseph's The Worker Childhood Center | 2105 St Joseph Street, Dubuque, IA 52001 | Center |
| St Mark Community Center | 1201 Locust Street, Dubuque, IA 52001 | Center |
| St Marys School Preschool | 701 Il Route 35 N, East Dubuque, IL 61025 | Preschool |
| University of Dubuque Child Care Center | 1001 McCormick St, Dubuque, IA 52001 | Center |
| Western Dubuque CSD - Seton Rainbow Circle Preschool | 10801 Sundown Road, Peosta, IA 52068 | Center |
| Young-Uns Preschool & Child Care Center | 3375 Kennedy Circle, Dubuque, IA 52001 | Center |