| Blacksburg KinderCare | 844 University City Blvd, Blacksburg, VA 24060 | Center |
| Blacksburg South KinderCare | 1301 Rugby Lane, Blacksburg, VA 24060 | Center |
| Rainbow Early Learning Center | 1511 Tyler Ave, Radford, VA 24141 | Center |
| Adventure Club of Montgomery County - Belview | 3187 Pepper's Ferry Road, Radford, VA 24141 | Center |
| Adventure Club of Montgomery County - Falling Branch | 735 Falling Branch Road, Christiansburg, VA 24073 | Center |
| Adventure Club of Montgomery County - Kipps | 2801 Price's Fork Road, Blacksburg, VA 24060 | Center |
| Adventure Club of Montgomery County - Price's Fork | 4237 Price's Fork Road, Price's Fork Elementary, Blacksburg, VA 24060 | Center |
| Adventure Club of Montgomery County at Christiansburg Primary | 240 Betty Lane, Christiansburg, VA 24073 | Center |
| Adventure Club of Montgomery County at Margaret Beeks Elementary School | 709 Airport Road, Blacksburg, VA 24060 | Center |
| Blacksburg Montessori School | 120 Church Street, Blacksburg, VA 24060 | Center |
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| Blacksburg New School | 2500 North Main Street, Blacksburg, VA 24060 | Center |
| Bridges and Blossoms Preschool | 880 Life Drive, Christiansburg, VA 24073 | Preschool |
| Christiansburg Child Care Center II | 970 Radford Street, Christiansburg, VA 24073 | Center |
| Early Challenges Child Care Center | 364 Arbor Drive, Christiansburg, VA 24073 | Preschool |
| Kids Heaven Childcare and Learning Center | 106 B Southpark Drive, Blacksburg, VA 24060 | Preschool |
| New River Community Action Head Start Program - Blacksburg | 701 Church Street, Blacksburg, VA 24060 | Preschool |
| New River Community Action Head Start Program - Christiansburg | 135 Church Street, Christiansburg, VA 24073 | Preschool |
| Pathway Christian Academy | 1550 Roanoke St, Christiansburg, VA 24073 | Preschool |
| Radford Early Learning Center | 1511 Tyler Ave, Radford, VA 24141 | Center |
| Rainbow Riders Childcare Center | 1800 Research Center Drive, Blacksburg, VA 24060 | Center |
| Rainbow Riders Childcare Center | 307 North Knollwood Drive, Blacksburg, VA 24060 | Center |
| St. John Neumann Academy | 2470 Ramble Road, Blacksburg, VA 24060 | Preschool |
| St. Mary's Little Angels Preschool | 1205 Old Mill Road, Blacksburg, VA 24060 | Preschool |
| Tall Oaks Montessori School | 703 Tall Oaks Drive, Blacksburg, VA 24060 | Preschool |
| The Adventure Club Center | 844 University City Blvd, Blacksburg, VA 24060 | Preschool |
| The Adventure Club of Montgomery County at Harding Elementary School | 429 Harding Avenue, Blacksburg, VA 24060 | Preschool |
| The Children's Garden Primary School | 1301 Gladewood Drive, Blacksburg, VA 24060 | Preschool |
| The Children's Nest Preschool and Daycare Center | 1155 Draper Road, Blacksburg, VA 24060 | Preschool |
| The Enrichment Center at Adventure World | 200 Midway Plaza Drive NW, Christiansburg, VA 24073 | Preschool |
| The Learning Ladder Child Development Center | 95 Patricia Lane, Christiansburg, VA 24073 | Preschool |
| Tomorrow's World Child Care Center | 2025 Sunset Drive, Christiansburg, VA 24073 | Center |
| Virginia Tech Child Development Center for Learning and Research | 140 Wallace Hall, Blacksburg, VA 24061 | Preschool |