| Gerald Adams Elementary Vpk | 3855 College Road, Key West, FL 33040 | Preschool |
| Grace Lutheran School | 2713 Flagler Avenue, Key West, FL 33040 | Center |
| Inez Martin Child Care Center | 1100 Varela Street, Key West, FL 33040 | Preschool |
| Key West Preschool Co-opertive | 2610 Flagler Ave, Key West, FL 33040 | Preschool |
| Lighthouse Christian Academy | 5580 Macdonald Ave, Key West, FL 33040 | Center |
| Montessori Children's School of Key West | 1221 Varela Street, Key West, FL 33040 | Center |
| Poinciana Elementary School Vpk | 1407 Kennedy Drive, Key West, FL 33040 | Preschool |
| Poincianna Elementary Preschool | 1407 Kennedy Drive, Key West, FL 33040 | Preschool |
| Reynolds School Vpk | 1316 Reynolds Street, Key West, FL 33040 | Preschool |
| Sigsbee Preschool | 939 Felton Road, Key West, FL 33040 | Preschool |
|
| Sunbeam Christian School | 1311 Fifth Street, Key West, FL 33040 | Preschool |
| The Learning Center of Key West | 241 Trumbo Rd, Key West, FL 33040 | Center |