| ABC Day Care | 208 Majestic Circle, Greeneville, TN 37743 | Center |
| Asbury Child Enrichment Center | 205 South Main St., Greeneville, TN 37743 | Preschool |
| Boys & Girls Club Of Greeneville & Greene County | 740 West Church Street, Greeneville, TN 37745 | Center |
| Camp Creek Head Start - pre-kindergarten | 2941 Camp Creek Road, Greeneville, TN 37743 | Preschool |
| Carolyn's Cottage | 694 Jimmy Johnston Road, Greeneville, TN 37743 | Center |
| Center For Technology Head Start - pre-kindergarten | 1121 Hal Henard Rd., Greeneville, TN 37743 | Preschool |
| Children's Center Of Holston Home | 119 Fairgrounds Circle, Greeneville, TN 37745 | Preschool |
| Chuckey Extended School Program | 1605 Chuckey Hwy., Chuckey, TN 37641 | Center |
| Chuckey Head Start - pre-kindergarten | 1605 Chuckey Highway, Chuckey, TN 37641 | Preschool |
| Chuckey Pre-kindergarten | 1605 Chuckey Highway, Chuckey, TN 37641 | Preschool |
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| Debusk Elementary - Extended School Program | 740 Debusk Road, Greeneville, TN 37743 | Center |
| Debusk Pre-kindergarten | 740 Debusk Road, Greeneville, TN 37743 | Preschool |
| Doak Elementary Extended School Program | 70 West Street, Greeneville, TN 37745 | Center |
| Doak Pre-kindergarten | 70 West Street, Greeneville, TN 37745 | Preschool |
| Eastview Elementary School- Extended School Program | 454 E. Bernard Avenue, Greeneville, TN 37743 | Center |
| Eastview Head Start - pre-kindergarten | 454 East Bernard Ave., Greeneville, TN 37745 | Preschool |
| George Clem Head Start | 310 Floral St., Greeneville, TN 37743 | Preschool |
| Gooseberry School | 290 New Hope Road, Greeneville, TN 37745 | Center |
| Greene County Christian Preschool | 9802 107 Cut Off, Greeneville, TN 37743 | Preschool |
| Greeneville Adventist Academy Pre-kindergarten | 305 Takoma Avenue, Greeneville, TN 37743 | Preschool |
| Hal Henard Elementary - Extended School Program | 425 East Vann Rd., Greeneville, TN 37743 | Center |
| Hal Henard Head Start - pre-kindergarten | 425 East Vann Rd., Greeneville, TN 37743 | Preschool |
| Highland Learn & Grow Academy | 208 N. Highland Ave., Greeneville, TN 37743 | Center |
| It's All About Kids Childcare | 1421 Tusculum Blvd., Greeneville, TN 37745 | Center |
| Little Blessings | 100 Emerald Road, Mosheim, TN 37818 | Center |
| Mosheim Elementary Extended School Program | 297 West School Street, Mosheim, TN 37818 | Center |
| Mosheim Head Start - pre-kindergarten | 229 West School Street, Mosheim, TN 37818 | Preschool |
| Nelson's Day Care | 154 Harlan St., Greeneville, TN 37745 | Center |
| Noah's Ark Day Care & Preschool | 1505 West Main Street, Greeneville, TN 37743 | Preschool |
| Ottway Head Start - pre-kindergarten | 2705 Ottway Road, Greeneville, TN 37743 | Preschool |
| Precious Angels Montessori School | 565 Park Lane, Greeneville, TN 37743 | Center |
| Small Miracles | 2001 West Main Street, Greeneville, TN 37743 | Center |
| South Central Elementary School - SACC | 2955 Highway 107, Chuckey, TN 37641 | Center |
| South Central SACC | 2955 Highway 107, Chuckey, TN 37641 | Center |
| T. H. Mcneese Education Center - Elp Idea 619 | 993 Hal Henard Road, Greeneville, TN 37743 | Preschool |
| The Academy Child Care | 408 North Hardin Street, Greeneville, TN 37745 | Center |
| The Learning Tree Childrens Center | 150 Marshall Lane, Greeneville, TN 37743 | Center |
| The Wooden Shoe | 437 East Bernard Avenue, Greeneville, TN 37745 | Center |
| Tiny Tots | 428 Massengail Road North, Greeneville, TN 37743 | Center |
| Towering Oaks Christian Preschool | 1985 Buckingham Road, Greeneville, TN 37745 | Preschool |
| Tusculum Preschool | 116 North Rufe Taylor Road, Greeneville, TN 37745 | Preschool |
| Tusculum View Elementary Extended School Program | 1725 Lafayette St, Greeneville, TN 37745 | Center |
| Tusculum View Head Start - pre-kindergarten | 1725 Lafayette Street, Greeneville, TN 37745 | Preschool |