| La Petite Academy of Lakeland | 1940 Glendale Street, Lakeland, FL 33803 | Preschool |
| All Saints Academy | 5001 State Road 540 West, Winter Haven, FL 33880 | Center |
| Alpha Bright Children Learning Center | 438 Wabash Ave N, Lakeland, FL 33813 | Center |
| Alpha Kid Care | 2900 Sr 540a East, Lakeland, FL 33813 | Center |
| Alpha Kid Care at Highland City | 5335 9th Street Se, Highland City, FL 33846 | Center |
| Alpha Kids Learning Center | 1013 Lakeshore Dr, Lakeland, FL 33803 | Preschool |
| Bartow Educational Center | 1987 Us Hwy 17 South, Bartow, FL 33830 | Preschool |
| Bartow Wee Care | 1570 South Gordon Avenue, Bartow, FL 33830 | Preschool |
| Bright Imaginations | 6075 South Florida Ave., Lakeland, FL 33813 | Preschool |
| CT&S Annointed Learning Center II | 2302 Haywood Street, Lakeland, FL 33801 | Preschool |
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| Calvary Child Care | 3800 Recker Highway, Winter Haven, FL 33880 | Center |
| Covenant Child Development Center | 210 E Poppell Drive, Lakeland, FL 33813 | Preschool |
| Creative Minds Academy II | 1802 Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Creative World Learning Center | 1390 Magnolia St, Bartow, FL 33830 | Center |
| Discovery Learning Academy | 3915 Old Highway 37, Lakeland, FL 33813 | Preschool |
| Eagle Lake Christian Preschool | 670 N Eagle Drive, Eagle Lake, FL 33839 | Center |
| Eagles Nest Academy | 450 West Main Street, Bartow, FL 33830 | Preschool |
| Early Childhood Academy George Jenkins | 6000 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| Early Childhood Kidcare | 590 S. Wilson Avenue, Bartow, FL 33830 | Center |
| Early Childhood Learning Center | 1703 Skinner Street, Lakeland, FL 33801 | Preschool |
| First Baptist Church of Highland City Preschool | 5410 Yarborough Lane, Lakeland, FL 33812 | Preschool |
| First Methodist School | 455 South Broadway Avenue, Bartow, FL 33830 | Center |
| Future Children Development Center | 2128 S Crystal Lake Dr, Lakeland, FL 33801 | Center |
| Gifts 4 Life Christian Academy | 630 Waldron Avenue, Bartow, FL 33830 | Center |
| Gloryland Express II | 490 West Davidson Street, Bartow, FL 33830 | Preschool |
| Goal Seekers and Eagles Before and After School Program | 4730 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| God's Tots Learning Center | 875 E. Main Street, Bartow, FL 33830 | Center |
| Hands-N-Hearts in Motion | 3330 Winterlake Road, Lakeland, FL 33803 | Preschool |
| Haven Christian Academy | 2105 King Rd Sw, Winter Haven, FL 33880 | Center |
| Heavenly Kids Christian Academy | 2605 Sunshine Drive South, Lakeland, FL 33801 | Center |
| Henderson Children of Glory Childcare Center | 109 N 3rd St, Eagle Lake, FL 33839 | Center |
| Highlands Christian Academy | 4210 Lakeland Highlands Rd., Lakeland, FL 33813 | Center |
| Highways To Tomorrow | 801 N Broadway Ave, Bartow, FL 33830 | Preschool |
| JC's Early Learning Center | 2540 S Crystal Lake Dr, Lakeland, FL 33801 | Preschool |
| Jeanni's Preschool and School Age | 905 Spirit Lake Rd, Winter Haven, FL 33880 | Center |
| Joyful Hearts Preschool a Ministry of Lakela | 1725 Parker Road, Lakeland, FL 33811 | Preschool |
| Just 4 Kids Preschool | 801 Nw 3rd Street, Mulberry, FL 33860 | Preschool |
| Just 4 Kids Preschool Phase II | 209 E Canal Street, Mulberry, FL 33860 | Preschool |
| Kids World Enrichment Center | 1860 East Gibbons St., Bartow, FL 33830 | Center |
| Kidz Life After School | 915 South Broadway, Bartow, FL 33830 | Center |
| Kidz R Kidz Early Education Center | 2203 Hwy 17 S, Bartow, FL 33830 | Center |
| Learning Tree Child Development Center | 2995 Avenue G Nw, Winter Haven, FL 33880 | Preschool |
| Little Footsteps of Lakeland | 6595 South Florida Avenue, Lakeland, FL 33813 | Preschool |
| Little Leaders of Tomorrow Child Care Center | 850 Forrest Drive, Bartow, FL 33830 | Preschool |
| Little World at Shepherd Road | 1333 Shepherd Rd, Lakeland, FL 33811 | Preschool |
| Medulla Baptist Church Preschool Learning Center | 3930 Old Hwy 37, Lakeland, FL 33813 | Center |
| Miss Adele's Learning Center | 1804 Richmond Road, Lakeland, FL 33803 | Preschool |
| Montessori Preschool of Lakeland | 334 Doris Dr, Lakeland, FL 33813 | Preschool |
| New Beginnings Preschool Learning Center | 5965 Sr 542 West, Winter Haven, FL 33880 | Preschool |
| Noah's Ark at United Methodist Temple | 2700 South Florida Avenue, Lakeland, FL 33803 | Preschool |
| Presbyterian Church in the Highlands | 1010 Lake Miriam Dr, Lakeland, FL 33813 | Preschool |
| R.E.A.L. Kidz Enrichment Center | 2195 E Georgia St, Bartow, FL 33830 | Center |
| RCMA Eloise Child Development Center | 710 Snively Ave, Winter Haven, FL 33880 | Preschool |
| RCMA Wahneta Child Development Center | 1920 Gerber Dairy Road, Winter Haven, FL 33880 | Preschool |
| Redeemer Lutheran Christian Preschool | 585 E Summerlin St, Bartow, FL 33830 | Center |
| Richardson's Learning Enrichment Center | 1426 W Bryant St, Bartow, FL 33830 | Preschool |
| SACCA Boswell | 2820 K-ville Avenue, Auburndale, FL 33823 | Center |
| SACCA Jewett Kid Care | 2250 8th St Ne, Winter Haven, FL 33880 | Center |
| SACCA Lake Shipp Kid Care | 250 Camellia Dr, Winter Haven, FL 33880 | Center |
| SACCA Scott Lake Kid Care | 1140 E Sr 540a, Lakeland, FL 33813 | Center |
| Safe and Sound Child Care and Early Learning Center | 580 East Laurel Ave, Eagle Lake, FL 33839 | Preschool |
| Scott Lake Christian Preschool | 5811 Scott Lake Rd, Lakeland, FL 33813 | Center |
| Shepherd Road Christian Academy | 1217 Shepherd Road, Lakeland, FL 33811 | Center |
| Small Wonders Preschool | 4777 Lakeland Highlands Road, Lakeland, FL 33813 | Center |
| Smart Start Youth & Families Services | 2727 Shepherd Rd, Lakeland, FL 33811 | Center |
| Smart Start Youth & Families Services | 4510 Lakeland Highlands Rd, Lakeland, FL 33813 | Center |
| Smart Start Youth & Family Services | 328 E Edgewood Drive, Lakeland, FL 33803 | Center |
| Smart Start Youth & Family Services | 6336 Cypress Gardens Blvd, Winter Haven, FL 33880 | Preschool |
| Smith Child Care Center | 402 Nw 9th Avenue, Mulberry, FL 33860 | Preschool |
| Southside Learning Center | 4820 Cleveland Heights Blvd., Lakeland, FL 33813 | Preschool |
| St John Neumann Preschool | 501 E Carter Road, Lakeland, FL 33813 | Preschool |
| St. Davids Clubhouse | 145 East Edgewood Drive, Lakeland, FL 33803 | Center |
| St. Paul Lutheran School | 4450 Harden Boulevard, Lakeland, FL 33813 | Center |
| Taylor Learning Center | 827 White Lane, Lakeland, FL 33813 | Preschool |
| The Brightest Minds Academy Bartow | 1150 Martin Luther King Blvd, Bartow, FL 33830 | Center |
| Traviss Teen Parent Program | 3225 Winter Lake Rd., Lakeland, FL 33803 | Center |
| Treehouse Learning & Enrichment Center | 2025 Bartow Rd., Lakeland, FL 33801 | Preschool |
| Treehouse Learning Center | 2025 Bartow Road, Lakeland, FL 33801 | Center |
| Trinity Learning Center | 500 W Stuart Street, Bartow, FL 33830 | Center |
| Unlimited Potential 1 | 1105 E Martin Luther King Blvd, Bartow, FL 33830 | Preschool |
| Unlimited Potential Child Care Center | 710 South Second Avenue, Bartow, FL 33830 | Center |
| Xtreme Kids Lite | 5001 State Road 540 West, Winter Haven, FL 33880 | Center |
| YMCA - Activity Club | 3620 Cleveland Heights Blvd, Lakeland, FL 33803 | Center |
| YMCA - Southwest Campus Kid Care | 2650 Southwest Ave, Lakeland, FL 33803 | Center |
| YMCA - Stephens Campus Kid Care | 1350 North Maple Avenue, Bartow, FL 33830 | Center |
| YMCA Spessard Holland Campus Kid Care | 2342 E.f.griffin Road, Lakeland, FL 33803 | Center |