| A Childs Place Inc | 8325 Old Lemay Ferry Rd, Barnhart, MO 63012 | Center |
| Adventures In Learning -desoto | 1107 Clarke St, De Soto, MO 63020 | Center |
| All Aboard Learning Center | 544 Karen Dr, Hillsboro, MO 63050 | Center |
| Alpha and Omega Child Care II | 429 Main St, Pevely, MO 63070 | Center |
| Angels In Joyland | 450 Bailey Rd, Crystal City, MO 63019 | Preschool |
| Bright Beginning Learning Center | 1549 W Main St, Festus, MO 63028 | Center |
| Children of Destiny Child Care | 15533 Gamel Cemetery Rd, Festus, MO 63028 | Center |
| Childrens House of Hillsboro | 603 Maple St, Hillsboro, MO 63050 | Center |
| Daytime Discoveries | Po Box 275, Valmeyer, IL 62295 | Center |
| Immanuel Lutheran Christian Childcare and Preschool | 19 N 3rd St, Festus, MO 63028 | Preschool |
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| Jefferson College Child Development Center | 1000 Viking Dr, Hillsboro, MO 63050 | Preschool |
| Jefferson R -7 Preschool | 2400 R-7 School Rd, Festus, MO 63028 | Preschool |
| Jefferson R7 Kids Korner | 2400 R-7 School Rd, Festus, MO 63028 | Center |
| Lil Thinkers Big Thinkers, Inc | 102 Fourth Street, Hillsboro, MO 63050 | Center |
| Lindsey Dawn Davis | 1610 Essex Heights Dr, De Soto, MO 63020 | Center |
| Little Scholars Preschool | 409 Deer Crossing Dr, Festus, MO 63028 | Preschool |
| Lori Collop Dulaney | 109 Williamsburg Dr, Crystal City, MO 63019 | Center |
| Mustard Seed Preschool | 6439 Highway 61-67, Imperial, MO 63052 | Preschool |
| Osceola Migrant Head Start Center | Hwy 61 North, Osceola, AR 72370 | Center |
| Peace Tabernacle Daycare | 11096 Highway 21, Hillsboro, MO 63050 | Center |
| Redeemer Preschool | 1620 New Boyd St, De Soto, MO 63020 | Preschool |
| Sacred Heart's Ursuline Learning Center | 201 Brierton Ln, Festus, MO 63028 | Center |
| Small Wonders Child Care Center | 10850 Highway 21, Hillsboro, MO 63050 | Preschool |
| St Lukes Preschool | 1101 Sulphur Springs Rd, Ballwin, MO 63021 | Preschool |
| The Playground Child Care Center | 1302 Kenner St, Crystal City, MO 63019 | Center |
| Wee Care Lc | 821 American Legion Dr, Festus, MO 63028 | Preschool |
| YMCA Jefferson County Athena Elementary | 3775 Athena School Rd, De Soto, MO 63020 | Center |
| YMCA Jefferson County Branch Festus | 1500 Mid Meadows Dr, Festus, MO 63028 | Center |
| YMCA Jefferson County Branch Our Ladys | 1550 St Mary's Ln, Festus, MO 63028 | Center |
| YMCA Jefferson County Crystal City Elementary | 600 Mississippi, Crystal City, MO 63019 | Center |
| YMCA of Jefferson County Dunklin R-v | 30 Main St, Pevely, MO 63070 | Center |
| YMCA of Jefferson County Hillsboro | 101 Leon Hall Pky, Hillsboro, MO 63050 | Center |
| YMCA of Jefferson County Lil Rascals Preschool | 1303 Ymca Dr, Festus, MO 63028 | Preschool |