| Apple Country Head Start - Tri County OIC - Senseny Road Center | 1481 Senseny Road, Winchester, VA 22602 | Preschool |
| Apple Country Head Start Clarke County Center - Boyce Elementary | 119 W. Main Street, Boyce, VA 22620 | Preschool |
| Apple Country Head Start at Douglas Learning Center | 598 N. Kent Street, Winchester, VA 22601 | Preschool |
| Apple Valley Montessori School | 1905 Henry Avenue, Winchester, VA 22601 | Preschool |
| Braddock Street United Methodist Early Learning Center | 115 Wolfe Street, Winchester, VA 22601 | Center |
| Building Blocks Childcare | 2170 S. Loudoun Street, Winchester, VA 22601 | Center |
| Children of America - Winchester | 631 W. Jubal Early Drive, Winchester, VA 22601 | Preschool |
| Children of America Stephens City | 201 Centre Drive, Suite 101, Stephens City, VA 22655 | Preschool |
| Cuddle Bears Day Care Center | 341 Sunnyside Plaza Circle, Winchester, VA 22603 | Center |
| Fremont Street Nursery | 533 Fremont Street, Winchester, VA 22601 | Preschool |
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| Precious One's Preschool and Daycare | 817 North Loudoun Street, Winchester, VA 22601 | Center |
| Sacred Heart Academy | 110 Keating Drive, Winchester, VA 22601 | Preschool |
| Sharon's Centre for the Education of Young Children | 1855 Senseny Road, Winchester, VA 22602 | Center |
| Sharon's Centre for the Education of Young Children | 255-3 Fairfax Pike, Stephens City, VA 22655 | Center |
| Shenandoah University Child Care Center | 118 Regency Lakes Drive, Winchester, VA 22603 | Center |
| Sneakers Educational Childcare | 915 S. Cameron Street, Winchester, VA 22601 | Preschool |
| TLC Daycare Center | 32 Weems Lane, Winchester, VA 22601 | Center |
| The Children's Enrichment Program | 75 Merriman's Lane, Winchester, VA 22601 | Center |
| The Life Church Daycare | 201 Agape Way, Stephens City, VA 22655 | Center |
| The Tot Spot | 1597 Senseny Road, Winchester, VA 22602 | Center |
| Valley Health Child Care Center | 1842 Amherst Street, Winchester, VA 22601 | Preschool |
| Winchester Academy | 2400 Roosevelt Boulevard, Winchester, VA 22601 | Preschool |
| Winchester Day Nursery | 133 Lincoln Street, Winchester, VA 22601 | Preschool |
| Winchester Montessori School | 1090 W. Parkins Mill Road, Winchester, VA 22602 | Preschool |