| La Petite Academy of North Port | 2700 Bobcat Village Center Road, North Port, FL 34288 | Center |
| A Child's Place | 2200 Education Avenue, Punta Gorda, FL 33950 | Preschool |
| Academy for Early Learning at Port Charlotte | 2202 Lakeshore Circle, Port Charlotte, FL 33952 | Preschool |
| Academy of Little Achievers | 4344 Pinnacle Street, Punta Gorda, FL 33980 | Preschool |
| Building Blocks Child Care | 2555 N Toledo Blade Blvd, North Port, FL 34289 | Center |
| Busy Bee Early Learning Center | 329 Allen Street, Punta Gorda, FL 33950 | Preschool |
| Charlotte County Public Schools Early Head Start | 311 E Charlotte Avenue, Punta Gorda, FL 33950 | Preschool |
| Charlotte Regional YMCA Child Development Center | 733 E Olympia Avenue, Punta Gorda, FL 33950 | Preschool |
| Children's Christian Center | 2733 Tamiami Trail, Port Charlotte, FL 33952 | Preschool |
| Deep Creek Elementary Preschool | 26900 Harborview Road, Punta Gorda, FL 33980 | Preschool |
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| Early Birds Preschool & Day Care Center | 3154 Sunrise Trail, Port Charlotte, FL 33952 | Preschool |
| East Elementary Preschool | 27050 Fairway Drive, Punta Gorda, FL 33982 | Preschool |
| Edgewater YMCA Child Development Center | 22416 Glass Lane, Punta Gorda, FL 33980 | Preschool |
| Educare Learning Center | 3028 Caring Way Suite B, Port Charlotte, FL 33952 | Preschool |
| Formative Years Early Learning Center | 1291 Capricorn Boulevard, Punta Gorda, FL 33983 | Preschool |
| Franz Ross YMCA Child Developement Center | 19333 Quesada Avenue, Port Charlotte, FL 33948 | Preschool |
| Genesis Christian School | 19150 Helena Avenue, Port Charlotte, FL 33948 | Preschool |
| Good Shepherd Day School | 1800 Shreve Street, Punta Gorda, FL 33950 | Preschool |
| Good Shepherd Day School | 401 W Henry Street, Punta Gorda, FL 33950 | Center |
| Grace Community School of Port Charlotte | 4334 Laura Street, Port Charlotte, FL 33952 | Preschool |
| HOPE Child Care Center | 18150 Murdock Circle, Port Charlotte, FL 33948 | Center |
| J.A.M. CLUB | 24038 Harborview Road, Punta Gorda, FL 33980 | Center |
| Joyful Noise | 24515 Rampart Boulevard, Punta Gorda, FL 33980 | Preschool |
| Kingsway Elementary Preschool | 23300 Quasar Blvd, Punta Gorda, FL 33980 | Preschool |
| Liberty Elementary Preschool | 370 Atwater Street, Port Charlotte, FL 33954 | Preschool |
| Lots of Tots Learning Center | 4021 Gardner Drive, Port Charlotte, FL 33952 | Preschool |
| Meadow Park Elementary School Preschool | 3131 Lakeview Boulevard, Port Charlotte, FL 33948 | Preschool |
| Morning Star Child Care Center # 2 | 4200 Juniper Street, Port Charlotte, FL 33948 | Center |
| Morning Star Childcare Center and Preschool | 23455 Quasar Boulevard, Punta Gorda, FL 33980 | Preschool |
| Neil Armstrong Elementary Preschool | 22485 Foote Avenue, Port Charlotte, FL 33952 | Preschool |
| New Operation Cooper Street | 650 Mary Street, Punta Gorda, FL 33950 | Center |
| Our Little World Preschool | 1603 Castlerock Lane, Port Charlotte, FL 33948 | Preschool |
| Palm Christian Academy | 390 Flamingo Boulevard, Port Charlotte, FL 33954 | Center |
| Peace River Elementary School Preschool | 22400 Hancock Avenue, Punta Gorda, FL 33980 | Preschool |
| Port Charlotte United Methodist | 21075 Quesada Avenue, Port Charlotte, FL 33952 | Preschool |
| Pumpkin Patch Preschool | 18150 Murdock Circle, Port Charlotte, FL 33948 | Preschool |
| Punta Gorda Child Development Center | 10169 Tamiami Trail, Punta Gorda, FL 33950 | Preschool |
| Rainbow's End | 2036 Loveland Blvd, Punta Gorda, FL 33980 | Preschool |
| Rainbow's End Play and Learn Child Care Center | 2100 Loveland Boulevard, Punta Gorda, FL 33980 | Center |
| Sallie Jones Elementary School Preschool | 1221 Cooper Street, Punta Gorda, FL 33950 | Preschool |
| Small World Christian Learning Center | 2230 Hariet Street Northeast, Port Charlotte, FL 33952 | Preschool |
| Storybook Academy | 25170 E Marion Avenue, Punta Gorda, FL 33950 | Center |
| Storybook Too | 272 E Virginia Avenue, Punta Gorda, FL 33950 | Preschool |
| Tiny Hands Childcare Preschool | 20150 Midway Blvd, Port Charlotte, FL 33952 | Preschool |
| True Holiness Child Care | 326 E Charlotte Avenue, Punta Gorda, FL 33950 | Center |
| Universal Performing Arts Learning Center | 640 Tamiami Trail, Port Charlotte, FL 33953 | Preschool |
| YMCA Afterschool at Charlotte Harbor Center | 22450 Hancock Ave, Punta Gorda, FL 33980 | Center |
| YMCA Afterschool at Deep Creek | 26900 Harborview Road, Punta Gorda, FL 33983 | Center |
| YMCA Afterschool at East | 27050 Fairway Drive, Punta Gorda, FL 33950 | Center |
| YMCA Afterschool at Kingsway | 23300 Quasar Blvd, Port Charlotte, FL 33948 | Center |
| YMCA Afterschool at Neil Armstrong Elementary | 22100 Breezeswept Avenue, Port Charlotte, FL 33948 | Center |
| YMCA Afterschool at Sallie Jones | 1230 Narranja Street, Punta Gorda, FL 33950 | Center |