| Childtime of Orange Park | 1510 Business Center Drive, Fleming Island, FL 32003 | Preschool |
| Eagle Harbor Parkway KinderCare | 1735 Eagle Harbor Pkwy, Fleming Island, FL 32003 | Preschool |
| La Petite Academy of Middleburg | 3150 County Road 220, Middleburg, FL 32068 | Preschool |
| Annunciation Catholic School | 1610 Blanding Blvd., Middleburg, FL 32068 | Preschool |
| Asbury Learning Center | 2781 Henley Road, Green Cove Springs, FL 32043 | Preschool |
| Backpack University | 400 College Drive, Middleburg, FL 32068 | Center |
| Bannerman Learning Center | 608 Mill Street, Green Cove Springs, FL 32043 | Preschool |
| Blue Angels Child Development Center Learning | 2025 Hwy 16 West, Green Cove Springs, FL 32043 | Preschool |
| Celebration Children's Development Center | 806 Oak Street, Green Cove Springs, FL 32043 | Center |
| Doctors Inlet Elementary Preschool | 2634 Cr 220, Middleburg, FL 32068 | Preschool |
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| Episcopal Children's Services Early Learning Center | 2506 Blanding Boulevard, Middleburg, FL 32068 | Preschool |
| Episcopal Children's Services Early Learning Center | 1107 Martin Luther King Jr Boulevard, Green Cove Springs, FL 32043 | Preschool |
| First Class Learning Center | 939 Blanding Blvd., Orange Park, FL 32065 | Preschool |
| Fleming Island Elementary School Preschool | 4425 Lakeshore Drive, Fleming Island, FL 32003 | Preschool |
| Fox Meadows Chrisitian Daycare | 3227 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Gingerbread Learning Center | 30 Knight Boxx Road, Orange Park, FL 32065 | Preschool |
| Good Samaritan Learning Tree | 3813 Old Jennings Road, Middleburg, FL 32068 | Preschool |
| Hickory Grove Early Learning Center | 310 S. Oakridge Ave., Green Cove Springs, FL 32043 | Preschool |
| Ivy League Academy | 2245 Plantation Center Drive Suite 60, Fleming Island, FL 32003 | Preschool |
| Keystone Heights Elementary School Preschool | 335 S. Pecan Street, Orange Park, FL 32065 | Preschool |
| KidsFirst Learning Center | 2645 Blanding Blvd, Middleburg, FL 32068 | Preschool |
| Lake Asbury Elementary Preschool | 2901 Sandridge Rd, Green Cove Springs, FL 32043 | Preschool |
| Little Paws Learning Center | 466 Madison Avenue, Orange Park, FL 32065 | Preschool |
| Little People's Learning Center | 5095 Lakeshore Drive West, Fleming Island, FL 32003 | Preschool |
| Mulberry Lane Childcare | 57 College Drive, Orange Park, FL 32065 | Preschool |
| O.P.K. South of Middleburg | 3425 Habitat Drive, Middleburg, FL 32068 | Center |
| O2B Kids 7 | 1821 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| O2B Kids 7 | 1821 Town Center Blvd. Suite 101, Fleming Island, FL 32003 | Preschool |
| Oakleaf Preschool & Childcare | 415 Meldrum Lane, Orange Park, FL 32065 | Preschool |
| Ollie's Day Care | 158 College Drive, Orange Park, FL 32065 | Preschool |
| Orange Park Preschool and Learning Center | 99 College Drive, Orange Park, FL 32065 | Preschool |
| Pinewood Christian Academy | 198 Knight Boxx Rd, Middleburg, FL 32068 | Preschool |
| Primrose School of Fleming Island | 2031 Town Center Boulevard, Fleming Island, FL 32003 | Preschool |
| Rideout Elementary Preschool | 3065 Apalachicola Blvd, Middleburg, FL 32068 | Preschool |
| Shadowlawn Elementary School Preschool | 2945 County Road 218, Green Cove Springs, FL 32043 | Preschool |
| Sharing Tree Preschool at Fleming Island | 5900 Highway 17 South, Fleming Island, FL 32003 | Preschool |
| St. Margaret's Children's House | 6865 Pine Avenue, Fleming Island, FL 32003 | Preschool |
| Sugar N Spice | 3211 Moody Avenue, Orange Park, FL 32065 | Preschool |
| Tender Hearts Child Care | 29 Cinnamon Street, Middleburg, FL 32068 | Center |
| The Goddard School of Fleming Island | 2280 Village Square Parkway, Fleming Island, FL 32003 | Preschool |
| Tynes Elementary Preschool | 1550 Tynes Blvd, Middleburg, FL 32068 | Preschool |
| Wee Care DayCare & Preschool, Inc | 4717 County Road 218, Middleburg, FL 32068 | Preschool |
| Wilkinson Elementary School Preschool | 4965 County Road 218, Middleburg, FL 32068 | Preschool |
| YMCA Pryme Time at C.E. Bennett | 1 South Oakridge Avenue, Green Cove Springs, FL 32043 | Center |
| YMCA Pryme Time at Coppergate Elementary | 2250 County Road 209 North, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Drs. Inlet Elementary | 2634 State Road 220, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Fleming Island Elementary | 4425 Lake Shore Drive, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at JL Wilkinson | 4965 County Highway 218, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Lake Asbury Elementary | 2901 Sandridge Road, Green Cove Springs, FL 32043 | Center |
| YMCA Pryme Time at Middleburg Elementary | 3958 Main Street, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Paterson Elementary | 5400 Pine Avenue, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Rideout Elementary | 3065 Apalachicola Boulevard, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Ridgeview Elementary | 421 Jefferson Ave., Orange Park, FL 32065 | Center |
| YMCA Pryme Time at Shadowlawn Elementary | 2945 County Road 218, Green Cove Springs, FL 32043 | Center |
| YMCA Pryme Time at Swimming Pen Creek | 1630 Woodpecker Lane, Middleburg, FL 32068 | Center |
| YMCA Pryme Time at Thunderbolt Elementary | 2020 Thunderbolt Road, Fleming Island, FL 32003 | Center |
| YMCA Pryme Time at Tynes Elementary | 1550 Tynes Boulevard, Middleburg, FL 32068 | Center |