| ACPC - Edwards | 3622 Woodland St, Ames, IA 50014 | Center |
| ACPC - Fellows | 1400 McKinley Dr, Ames, IA 50010 | Center |
| ACPC - Main Center | 611 Clark Ave, Ames, IA 50010 | Center |
| ACPC - Meeker | 300 20th St, Ames, IA 50010 | Center |
| ACPC - Mitchell | 3521 Jewel Drive, Ames, IA 50010 | Center |
| ACPC - Northminster Site | 1416 20th St, Ames, IA 50010 | Center |
| ACPC - Northwood | 3012 Duff Avenue, Ames, IA 50010 | Center |
| ACPC - Sawyer Site | 4316 Ontario St, Ames, IA 50010 | Center |
| ACPC - Toddler Center | 521 6th St, Ames, IA 50010 | Center |
| Adventures Preschool | 2622 W Lincoln Way, Ames, IA 50014 | Center |
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| Ames Christian School | 925 S 16th St, Ames, IA 50010 | Center |
| Bethesda Christian Preschool | 1517 Northwestern Ave, Ames, IA 50010 | Center |
| Childserve Child Care | 1915 Philadelphia St, Ames, IA 50010 | Center |
| Eagle's Loft Child Care Center | 2715 Dayton Ave, Ames, IA 50010 | Center |
| Formative Years | 4510 Todd Dr, Ames, IA 50010 | Center |
| ISU Child Dev Lab School | 4380 Palmer HDFS Bldg Ste 0351, Ames, IA 50011 | Center |
| Lil' Cubs Preschool | 224 S 6th St, Nevada, IA 50201 | Center |
| Luella Kalvik's Preschool | 418 Cedar St, Story City, IA 50248 | Center |
| MICA Head Start - Nevada | 910 10th St, Nevada, IA 50201 | Center |
| Ms. Hollie's Preschool | 905 N 5th Ave, Huxley, IA 50124 | Center |
| Nevada Community School District | Nevada Comm Rsrce Ctr, Nevada, IA 50201 | Center |
| Play and Learn Children's Center | 301 Alexander Ave Ste A, Ames, IA 50010 | Center |
| Prairie Flower Children's Center | 2807 Duff Ave, Ames, IA 50010 | Center |
| Small Wonders Preschool | 1500 Gateway Hills Park Dr, Ames, IA 50010 | Center |
| St Paul Lutheran Preschool - Ames | 610 15th St, Ames, IA 50010 | Center |
| The Caterpillar Clubhouse | 111 Main Street, Gilbert, IA 50105 | Center |
| Three C's Comm Center | 515 N. Main, Huxley, IA 50124 | Center |
| University Community Childcare | 100 University Village, Ames, IA 50010 | Center |
| Vet Med Child Care Center | 1700 Christensen Dr, Ames, IA 50010 | Center |
| YSS Gilbert Kids Club | 109 Rothmoor Drive, Gilbert, IA 50105 | Center |