| La Petite Academy of Kissimmee | 2825 Alice Boulevard, Kissimmee, FL 34746 | Preschool |
| 4 C Headstart Baker St | 3500 Baker Street, Kissimmee, FL 34741 | Preschool |
| 4 C Headstart Palm Plaza | 2234-2236 East Irlo Bronson Mem ..., Kissimmee, FL 34744 | Preschool |
| 4C Osceola Head Start Tennessee Ave | 1019 Tennessee Avenue, Saint Cloud, FL 34769 | Center |
| Academy For Kids | 2375 Canoe Creek Road, Saint Cloud, FL 34769 | Preschool |
| Actively Believing In Children | 1420 Louisiana Avenue, Saint Cloud, FL 34769 | Preschool |
| Canoe Creek Charter Preschool | 3600 Canoe Creek Road, Saint Cloud, FL 34772 | Preschool |
| Chestnut Elementary School Preschool | 4300 Chestnut Street, Kissimmee, FL 34759 | Preschool |
| City of Life Christian Academy | 2874 E Irlo Bronson Memorial Hwy, Kissimmee, FL 34744 | Preschool |
| Creative Learning Academy Center | 3301 Forest Drive, Kissimmee, FL 34746 | Preschool |
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| Deerwood Elementary School Preschool | 3701 Marigold Ave, Kissimmee, FL 34758 | Preschool |
| First United Methodist Church Child Care Center | 101 West Dakin Avenue, Kissimmee, FL 34741 | Preschool |
| First United Methodist School | 122 West Sproule Avenue, Kissimmee, FL 34741 | Preschool |
| Garden of Stars | 1901 South John Young Parkway, Kissimmee, FL 34741 | Preschool |
| God's Creation Childcare Center | 2242 East Irlo Bronson Memorial ..., Kissimmee, FL 34744 | Preschool |
| Grace Lutheran Early Learning Center | 1123 Louisiana Avenue, Saint Cloud, FL 34769 | Center |
| Hansel and Gretel 2 | 4513 Neptune Rd, Saint Cloud, FL 34769 | Preschool |
| Happy Feet Day Care and Learning Center | 2244 Boggy Creek Road Unit A & ..., Kissimmee, FL 34744 | Center |
| Kid's World Preschool & Child Care Center | 1202 Wisconsin Avenue, Saint Cloud, FL 34769 | Preschool |
| Kiddie Kollege | 715 Dakota Ave, Saint Cloud, FL 34769 | Center |
| Kids In The Country | 3836 Friars Cove Road, Saint Cloud, FL 34772 | Preschool |
| Kingdom Kids Christian Academy | 1003 South John Young Parkway, Kissimmee, FL 34741 | Preschool |
| Life Academy Preschool | 2269 Partin Settlement Road, Kissimmee, FL 34744 | Preschool |
| Little Lambs Preschool | 601 Walnut Street, Kissimmee, FL 34759 | Center |
| Living Water Child Development Center | 4101 Pleasant Hill Rd, Kissimmee, FL 34746 | Center |
| My Angel Dream Day Care | 3354 West Southport Road, Kissimmee, FL 34746 | Preschool |
| Noah's Ark FDCH | 735 Cockatoo Ct, Kissimmee, FL 34759 | Preschool |
| Pleasant Hill Academy | 2525 Trafalgar Blvd, Kissimmee, FL 34758 | Center |
| Poinciana 247 Preschool | 90 S Doverplum Avenue, Kissimmee, FL 34759 | Preschool |
| Poinciana Elementary School Preschool | 4201 Rhododendron Avenue, Kissimmee, FL 34758 | Preschool |
| Preschool Connection Academy | 2925 Canoe Creek Road, Saint Cloud, FL 34772 | Preschool |
| Reedy Creek Elementary School Preschool | 5100 Eagles Trail, Kissimmee, FL 34758 | Preschool |
| Rocking Horse Preschool | 1015 Tennessee Ave, Saint Cloud, FL 34769 | Preschool |
| Ross E Jeffries Elementary School Preschool | 1200 Vermont Ave, Saint Cloud, FL 34769 | Preschool |
| Southland Christian School Preschool | 2901 17th St, Saint Cloud, FL 34769 | Center |
| St Luke & St Peter's Preschool | 2745 Canoe Creek Road, Saint Cloud, FL 34772 | Center |
| Sunrise Elementary School Preschool | 1925 Ham Brown Road, Kissimmee, FL 34746 | Preschool |
| Thacker Avenue Elementary School Preschool | 301 North Thacker Ave, Kissimmee, FL 34741 | Preschool |
| YMCA Lake Marion Creek Campus Kidcare | 3055 Lake Marion Creek Dr, Kissimmee, FL 34759 | Center |
| Zenith Volunteery Pre-Kindergarten Program VPK | 2218 E. Irlo Bronson Memorial Hwy, Kissimmee, FL 34744 | Preschool |