| Challenger Head Start | 901 East Calle Meyer, Nogales, AZ 85621 | Preschool |
| Dinobones Preschool | 353 Planta Court, Rio Rico, AZ 85648 | Center |
| Montessori De Santa Cruz | 18 Calle Baca, Tubac, AZ 85646 | Center |
| Rain Forest Montessori Preschool & Daycare Center | 786 N Industrial Park, Nogales, AZ 85621 | Preschool |
| Rio Rico Head Start | 1412 West Frontage Road, Rio Rico, AZ 85648 | Preschool |
| S.C.V.U.S.D. #35 - Pena Blanca Preschool | 131 Camino Maricopa, Rio Rico, AZ 85648 | Preschool |
| S.C.V.U.S.D. #35 - San Cayetano Preschool | 1374 West Frontage Road, Rio Rico, AZ 85648 | Preschool |
| Small Steps Preschool | 1080 N Las Americas, Nogales, AZ 85621 | Preschool |
| Smart Kids | 939 North Perkins Avenue, Nogales, AZ 85621 | Center |
| Sonshine Christian Preschool | 1916 North Frank Reed Rd, Nogales, AZ 85621 | Preschool |
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| St. Andrew's Preschool & Day Care Center | 969 West Country Club Dr., Nogales, AZ 85621 | Preschool |
| Sunshine Preschool | 870 North Perkins, Nogales, AZ 85621 | Preschool |
| Western Head Start | 686 North Western Avenue, Nogales, AZ 85621 | Preschool |