| La Petite Academy of Deltona | 698 Deltona Blvd, Deltona, FL 32725 | Preschool |
| ABC Day Care & Preschool | 2855 Lake Helen - O'steen Road, Deltona, FL 32738 | Preschool |
| Alpha Learning Center | 2000 Howland Blvd, Deltona, FL 32738 | Preschool |
| Amanda's Childcare & Preschool | 123 W Rhode Island Rd, Orange City, FL 32763 | Preschool |
| Amanda's Childcare II | 115 W. Rhode Island Road, Orange City, FL 32763 | Center |
| Blake Memorial Dayschool | 134 N Euclid Avenue, Lake Helen, FL 32744 | Center |
| Boston Avenue Preschool | 340 N. Boston Avenue, Deland, FL 32724 | Preschool |
| Casa Montessori | 217 North Stone Street, Deland, FL 32720 | Preschool |
| Child's World of Learning | 2700 Enterprise Road, Orange City, FL 32763 | Preschool |
| Children First Academy | 825 S Spring Garden Avenue, Deland, FL 32720 | Preschool |
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| Children's House Montessori School | 509 E Pennsylvania Avenue, Deland, FL 32724 | Center |
| Children's Playskool | 1748 South Woodland Blvd., Deland, FL 32720 | Preschool |
| Christian Care for Lil' Angels | 74 S Charles R Beall Blvd, Debary, FL 32713 | Preschool |
| Coleman Head Start Center | 200 East Blue Springs, Orange City, FL 32763 | Preschool |
| Community United Methodist Preschool | 41 W. Highbanks Road, Debary, FL 32713 | Preschool |
| Cornerstone Christian Learning Center | 2813 Howland Blvd, Deltona, FL 32725 | Center |
| DTA Academy | 1649 Providence Blvd, Deltona, FL 32725 | Preschool |
| DeBary Sonshine Academy | 76 W. Highbanks Road, Debary, FL 32713 | Center |
| DeBary Sonshine Afterschool Program | 88 Highbanks Road, Debary, FL 32713 | Center |
| DeLand Family YMCA Childcare | 761 E Int'l Speedway Blvd, Deland, FL 32724 | Preschool |
| DebaryFirst Presbyterian Preschool | 267 E. Highbanks Road, Debary, FL 32713 | Preschool |
| Deland High School Infant - Toddler Development Center | 800 North Hill Avenue, Deland, FL 32724 | Center |
| Deltona Baptist Academy | 1651 Doyle Road, Deltona, FL 32725 | Center |
| Deltona Christian School | 1200 Providence Blvd, Deltona, FL 32725 | Center |
| Deltona United Methodist Preschool | 1045 E Normandy Blvd, Deltona, FL 32725 | Preschool |
| Easter Seals of Volusia & Flagler County | 2219 S Woodland Blvd, Deland, FL 32720 | Preschool |
| Emmaus Lutheran Preschool | 2500 S Volusia Ave, Orange City, FL 32763 | Preschool |
| First Baptist Early Childhood Development Center | 725 N Woodland Blvd, Deland, FL 32720 | Preschool |
| First Baptist Preschool Learning Center | 975 E Graves Avenue, Orange City, FL 32763 | Preschool |
| First Presbyterian Church Day School | 724 North Woodland Boulevard, Deland, FL 32720 | Preschool |
| First United Methodist Church of DeLand - Preschool | 115 East Howry Avenue, Deland, FL 32724 | Preschool |
| Florida United Methodist Children's Home Child Care Center | 51 Children's Way, Deltona, FL 32725 | Preschool |
| Forever Learning Center | 227 S. Florida Ave., Deland, FL 32720 | Center |
| Gingerbread Childcare | 138 E French Avenue, Orange City, FL 32763 | Preschool |
| Great Beginnings Learning Center | 337 Old Daytona Road., Deland, FL 32724 | Preschool |
| Heavenly Angelz Kidz Center | 950 Doyle Road, Deltona, FL 32725 | Center |
| Johnson Academy of Early Learning | 465 S. Volusia Avenue, Orange City, FL 32763 | Preschool |
| Keiki s Place | 2290 Florida Drive, Deltona, FL 32738 | Preschool |
| Kid's Korner Development Center | 1400 N Clara Avenue, Deland, FL 32720 | Preschool |
| Kiddie Koledge | 1114 W. Beresford Avenue, Deland, FL 32720 | Preschool |
| Kids Life! | 346 N. Volusia Avenue, Orange City, FL 32763 | Center |
| Kids with Grace Childcare | 767 Forth Smith Blvd. Suite 104, Deltona, FL 32738 | Preschool |
| Kidz Connection Learning Center | 1785 Elkcam Blvd, Deltona, FL 32725 | Center |
| Kidz Learn & Play | 541 S Volusia Avenue, Orange City, FL 32763 | Center |
| King's Kids Academy | 3185 Yorkshire Drive, Deltona, FL 32738 | Center |
| Learning Bridge Academy | 2411 East Graves Avenue, Deltona, FL 32725 | Preschool |
| Learning Bridge Academy | 2411 E. Graves Avenue, Orange City, FL 32763 | Center |
| Lighthouse Christian Academy - DeLand | 126 S Ridgewood Avenue, Deland, FL 32720 | Center |
| Lil Lambs Christian Pre School | 401 E. Taylor Road, Deland, FL 32724 | Center |
| Little Blessings Preschool | 1310 Maximillian Street, Deltona, FL 32725 | Preschool |
| Little Cherubs Learning Academy | 107 S Chandler Avenue, Deland, FL 32724 | Preschool |
| Little Discoveries Academy | 637 Deltona Blvd., Deltona, FL 32725 | Preschool |
| Malloy Head Start Center | 330 E Retta St, De Leon Springs, FL 32130 | Preschool |
| Mater Christi Preschool PSG | 50 Mansfield Avenue, West Greenwich, RI 02817 | Center |
| Merry Day Child Care Center | 407 E Kentucky Avenue, Deland, FL 32724 | Preschool |
| My Little Friends Preschool | 285 W Blue Springs Ave, Orange City, FL 32763 | Preschool |
| New Creation Academy | 1250 S. Spring Garden Avenue ..., Deland, FL 32720 | Preschool |
| New Horizons Babies & Toddlers | 227 S Florida Ave, Deland, FL 32720 | Center |
| New Horizons Discovery Centers | 500 S Dexter Avenue, Deland, FL 32720 | Preschool |
| Rainbow Child Care Center | 1401 W New York Avenue, Deland, FL 32720 | Preschool |
| Saint Barnabas Episcopal School | 322 West Michigan Avenue, Deland, FL 32720 | Preschool |
| Sandcastle Day Care & Preschool | 1382 Howland Blvd., Deltona, FL 32725 | Preschool |
| St. Barnabus Episcopal School | 322 West Michigan Avenue, Deland, FL 32720 | Preschool |
| Stepping Stones Child Care | 1575 Detrick Avenue, Deland, FL 32724 | Preschool |
| Stetson Baptist Christian School | 1025 W Minnesota Avenue, Deland, FL 32720 | Preschool |
| Sunshine Daycare and Preschool | 1696 Providence Blvd., Deltona, FL 32725 | Preschool |
| The Little House Next Door Child Development | 133 E. Church Street, Deland, FL 32720 | Preschool |
| Trinity Christian Academy Preschool | 875 Elkcam Boulevard, Deltona, FL 32725 | Preschool |
| Trinity Learning Center | 306 W Wisconsin Ave, Deland, FL 32720 | Preschool |
| Twinkle Star Center | 238 S Amelia Ave, Deland, FL 32724 | Preschool |
| Twinkle Toes Early Learning Center | 399 N Orange Avenue, Orange City, FL 32763 | Preschool |
| Wee Kare | 371 Main Street, Deltona, FL 32725 | Preschool |
| West Volusia Educational Center | 803 South Woodland Boulevard, Deland, FL 32720 | Preschool |
| YMCA Kids Club at Deltona Lakes Baptist | 2886 Elkcam Blvd, Deltona, FL 32725 | Center |