| Desoto Development Center | 2441 McIngvale, Hernando, MS 38632 | Center |
| Faith Preschool Academy Southaven | 820 Church Road East, Southaven, MS 38671 | Center |
| Future Star Learning Center | 5511 Hwy 51 North, Horn Lake, MS 38637 | Center |
| Hernando Baptist Kindergarten | 11 East Center Street, Hernando, MS 38632 | Center |
| Hernando Headstart | 1290 Oak Grove Rd., Hernando, MS 38632 | Center |
| Hernando Methodist Preschool | 1890 Mt. Pleasant Road, Hernando, MS 38632 | Center |
| Jump Start Academy | 6295 Hwy 51 N, Horn Lake, MS 38637 | Center |
| Kaco's Kids | 905 Magnolia Hills Cove, Hernando, MS 38632 | Center |
| Living Word Ministry | 6995 Starlanding Rd., Lake Cormorant, MS 38641 | Center |
| Lucy Jones Daycare | 4440 Oak Grove Drive, Hernando, MS 38632 | Center |
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| Miti Kids Childcare & Learning Center | 6285 Ridgewood Drive, Horn Lake, MS 38637 | Center |
| Southaven Community Church Daycare | 5340 Airways, Southaven, MS 38671 | Center |
| Southaven First United Methodist Preschool - Afterschool | 723 Starlanding Road, Nesbit, MS 38651 | Center |
| The Learning Tree Christian Preschool & Afterschool | 881 West Service Drive, Coldwater, MS 38618 | Center |
| YMCA Desoto Central Primary School | 3210 Getwell Road South, Southaven, MS 38672 | Center |
| YMCA Horn Lake Elementary | 6341 Ridgewood, Horn Lake, MS 38637 | Center |
| YMCA Horn Lake Intermediate | 6585 Horn Lake Road, Horn Lake, MS 38637 | Center |
| YMCA Lake Cormorant Elementary School | 3285 Wilson Mill Road, Lake Cormorant, MS 38641 | Center |
| YMCA Primetime Hernando Elementary | 455 Riley Road, Hernando, MS 38632 | Center |
| YMCA Primetime Hernando Hills Elementary School | 570 McIngvale, Hernando, MS 38632 | Center |
| YMCA Primetime Oak Grove Elementary | 893 Oak Grove Road, Hernando, MS 38632 | Center |
| YMCA Shadow Oaks Elementary | 3780 Shadow Oaks Parkway, Horn Lake, MS 38637 | Center |