| 123 Grow With Me Infant Toddler Center | 2727 Bayshore Drive, Naples, FL 34112 | Center |
| 123 Grow With Me Learning Center | 2737 Bayshore Drive, Naples, FL 34112 | Preschool |
| A Step Up at Golden Gate | 2925 Magnolia Pond Drive, Naples, FL 34116 | Center |
| ABC Academy Learning Center | 5043 Coronado Parkway, Naples, FL 34116 | Preschool |
| After School Programs South at Calusa | 4600 Santa Barbara Blvd, Naples, FL 34104 | Center |
| After School Programs South at Lely | 8125 Lely Cultural Parkway, Naples, FL 34113 | Center |
| After School Programs at Big Cypress | 3250 Golden Gate Blvd W, Naples, FL 34120 | Center |
| After School Programs at Golden Terrace | 2711 44th Terrace Sw, Naples, FL 34116 | Center |
| After School Programs at Lake Park | 1295 14th Avenue N, Naples, FL 34102 | Center |
| After School Programs at Manatee | 1880 Manatee Rd, Naples, FL 34114 | Center |
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| After School Programs at Mike Davis | 3215 Magnolia Pond Rd, Naples, FL 34116 | Center |
| After School Programs at Seagate | 650 Seagate Dr, Naples, FL 34103 | Center |
| Ana's Little Angels Inclusion Learning Center | 2400 Kirkwood Avenue, Naples, FL 34112 | Preschool |
| Avalon Elementary Preschool | 3300 Thomasson Drive, Naples, FL 34112 | Preschool |
| Bear's Den Preschool-Downtown Naples Hospital | 350 7th Street N, Naples, FL 34102 | Preschool |
| Bottles Blocks and Books | 2772 Santa Barbara Blvd, Naples, FL 34116 | Preschool |
| Child's Path Preschool | 3144 Santa Barbara Blvd, Naples, FL 34116 | Preschool |
| Collier Childcare Resources at Edison State | 7007 Lely Cultural Parkway, Naples, FL 34113 | Preschool |
| Creation Station Wee Christian Learning Center | 8000 Goodlette Road N, Naples, FL 34109 | Preschool |
| Creative Corner Preschool | 1397 13th Avenue N, Naples, FL 34102 | Center |
| East Naples Community Park | 3500 Thomasson Drive, Naples, FL 34112 | Center |
| East Naples United Methodist Church Learning Center | 2701 Airport Road S, Naples, FL 34112 | Preschool |
| First Baptist Academy | 3000 Orange Blossom Drive, Naples, FL 34109 | Center |
| First Presbyterian Preschool | 250 6th Street S, Naples, FL 34102 | Preschool |
| Fleischmann Park Community Center | 1600 Fleischmann Boulevard, Naples, FL 34102 | Center |
| Fun Time Early Childhood Academy | 102 12th Street North, Naples, FL 34102 | Preschool |
| Golden Gate Community Center | 4701 Golden Gate Parkway, Naples, FL 34116 | Preschool |
| Golden Gate Elementary Preschool | 4911 20th Place Southwest, Naples, FL 34116 | Preschool |
| Golden Gate High School Little Titans | 2925 Magnolia Pond Drive, Naples, FL 34116 | Preschool |
| Golden Terrace Elementary Preschool | 2711 44th Terrace Southwest, Naples, FL 34116 | Preschool |
| Grace Community School of Golden Gate | 5524 19th Court Sw, Naples, FL 34116 | Preschool |
| Grace Lutheran Preschool | 860 Banyon Blvd, Naples, FL 34102 | Preschool |
| Grizzlie Bears Child Care and Learning Center | 4405 Outer Drive, Naples, FL 34112 | Preschool |
| Here We Grow Child Development Center | 5269 Golden Gate Parkway, Naples, FL 34116 | Preschool |
| Huggie Bears Child Care and Learning Center | 4705 Outer Drive, Naples, FL 34112 | Preschool |
| Kiddie Korral On The Parkway | 3060 62nd Street Sw, Naples, FL 34116 | Preschool |
| LWIT Learning Partners Early Education | 3702 Estey Ave, Naples, FL 34104 | Center |
| Lambs of Faith Preschool | 4150 Goodlette Road N, Naples, FL 34103 | Preschool |
| Lely Elementary Preschool | 8125 Lely Cultural Parkway, Naples, FL 34113 | Preschool |
| Lely Presbyterian's St Andrew's Preschool | 105 St Andrews Blvd, Naples, FL 34113 | Preschool |
| Little Ducks Day School | 4025 10th Street North Unit 1&2, Naples, FL 34103 | Preschool |
| Louise Hasse Community Center | 3390 Golden Gate Blvd W, Naples, FL 34120 | Preschool |
| Manatee Elementary Preschool | 1880 Manatee Road, Naples, FL 34114 | Preschool |
| Methodist Day School | 388 First Ave South, Naples, FL 34102 | Preschool |
| Mike Davis Elementary Preschool | 3215 Magnolia Dr, Naples, FL 34116 | Preschool |
| Moorings Presbyterian Preschool | 791 Harbour Drive, Naples, FL 34103 | Preschool |
| Naples Adventist Christian School | 2629 Horseshoe Dr, Naples, FL 34104 | Preschool |
| Naples Christian Academy Preschool | 3161 Santa Barbara Boulevard, Naples, FL 34116 | Center |
| Naples Park Elementary Preschool | 685 - 111th Avenue North, Naples, FL 34108 | Preschool |
| Naples Preschool Academy | 1275 Airport Road S, Naples, FL 34104 | Preschool |
| Nicaea Academy | 2200 Santa Barbara Blvd, Naples, FL 34116 | Center |
| Osceola Elementary Preschool | 5470 Osceola Trail, Naples, FL 34109 | Preschool |
| Precious Cargo Academy | 5200 Crayton Road, Naples, FL 34103 | Preschool |
| RCMA Six L Child Development Center | 10355 Six L Farm Road, Naples, FL 34114 | Preschool |
| RCMA Youth Haven Child Development Center | 5867 Whitaker Road, Naples, FL 34112 | Preschool |
| Saint Ann Catholic School | 542 8th Ave S, Naples, FL 34102 | Preschool |
| Saint John the Evangelist Early Learning & Child Care | 625 111th Avenue N, Naples, FL 34108 | Center |
| Sandcastle Day Care | 100 Cape Sable Drive, Naples, FL 34104 | Preschool |
| Sandcastle Day Care | 6028 Radio Road #14, Naples, FL 34104 | Preschool |
| Shadowlawn Elementary Preschool | 2161 Shadowlawn Drive, Naples, FL 34112 | Preschool |
| So Big So Bright | 2960 South Horseshoe Drive #1000, Naples, FL 34104 | Preschool |
| Sports CLUB at Golden Gate | 4911 20th Place Sw, Naples, FL 34116 | Center |
| Sports CLUB at Osceola | 5770 Osceola Trail, Naples, FL 34109 | Center |
| Sports CLUB at Poinciana Elementary | 2825 Airport Road S, Naples, FL 34105 | Center |
| Sports CLUB at Shadowlawn | 2161 Shadowlawn Drive, Naples, FL 34112 | Center |
| Sports CLUB at Vineyards | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| St. Elizabeth Seton | 2760 52nd Terrace Sw, Naples, FL 34116 | Preschool |
| TOPS Academy | 1371 Shadowlawn Drive, Naples, FL 34104 | Preschool |
| The Children's House Learning Center | 2310 Hunter Blvd, Naples, FL 34116 | Preschool |
| The Creation Station Wee Christian Learning Center | 8000 Goodlette Rd N, Naples, FL 34109 | Preschool |
| The Salvation Army | 3180 Estey Avenue, Naples, FL 34104 | Center |
| The Village School | 6000 Goodlette Frank Road N, Naples, FL 34108 | Preschool |
| Vineyards Community Park | 6231 Arbor Blvd W, Naples, FL 34119 | Center |
| Vineyards Elementary Preschool | 6225 Arbor Boulevard West, Naples, FL 34119 | Preschool |
| Windmill Creek Academy | 2682 South Horseshoe Court, Naples, FL 34104 | Preschool |
| YMCA Child Development Center | 5450 Ymca Road, Naples, FL 34109 | Preschool |
| YMCA of the Palms Gaynor Child Development | 2510 Museum Drive, Naples, FL 34109 | Preschool |