| Amelia Island Montessori School | 1423 Julia Street, Fernandina Beach, FL 32034 | Center |
| Amelia Island Parent Cooperative Preschool | 5040 First Coast Highway, Fernandina Beach, FL 32034 | Center |
| Early Impressions Development Center | 112 S. 3rd Street, Fernandina Beach, FL 32034 | Preschool |
| Faith Christian Academy of NE Florida | 96282 Brady Point Road, Fernandina Beach, FL 32034 | Center |
| Fernandina Beach Recreation Department | 2500 Atlantic Avenue, Fernandina Beach, FL 32034 | Center |
| Island Academy | 1336 South 14th Street, Fernandina Beach, FL 32034 | Preschool |
| Jack and Jill Preschool | 416 Alachua Street, Fernandina Beach, FL 32035 | Center |
| La Petite Academy | 2120 Will Hardee Drive, Fernandina Beach, FL 32034 | Preschool |
| Lamb Day Care | 601 Centre Street, Fernandina Beach, FL 32034 | Preschool |
| Miss Kate's Pre K | 1303 Jasmine Street, Fernandina Beach, FL 32034 | Preschool |
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| Peck Head Start | 516 South 10th Street Suite 100, Fernandina Beach, FL 32034 | Preschool |
| St. Michael Academy Preschool | 510 Calhoun St, Fernandina Beach, FL 32034 | Preschool |
| Step by Step Learning Center I | 1986 Citrona Drive, Fernandina Beach, FL 32034 | Preschool |
| Step by Step Learning Center II | 95734 Amelia Concourse, Fernandina Beach, FL 32034 | Preschool |
| Thompson's Day Care | 216 South 13th Street, Fernandina Beach, FL 32034 | Center |
| YMCA Child Development Center at Yulee | 86029 Pages Dairy Road, Yulee, FL 32097 | Center |
| YMCA Pryme Time at Atlantic Kids Campus | 1205 Atlantic Avenue, Fernandina Beach, FL 32034 | Center |
| YMCA Pryme Time at Yulee Kid's Campus | 86029 Pages Dairy Road, Yulee, FL 32097 | Center |
| Yulee Baptist VPK Center | 85971 Harts Road, Yulee, FL 32097 | Preschool |
| Yulee Baptist Weekday Ministry | 85967 Harts Road, Yulee, FL 32097 | Center |