| Academy Road Country Care | 270 Academy Road, Walterboro, SC 29488 | Center |
| Alpha Wee Care Center | 318 Peurifoy Road, Walterboro, SC 29488 | Center |
| Bettys Tots | 1303 Rivers Street, Walterboro, SC 29488 | Center |
| Colleton County Academy | 229 Gruber Street, Walterboro, SC 29488 | Center |
| Forest Hills Preschool | 628 Hiers Corner Road, Walterboro, SC 29488 | Center |
| Little Bloomers Preschool | 281 Welch Creek Road, Walterboro, SC 29488 | Center |