| Beville Road KinderCare | 1320 Beville Rd, Daytona Beach, FL 32114 | Preschool |
| La Petite Academy of Port Orange | 650 Taylor Road, Port Orange, FL 32127 | Preschool |
| A Kids Place Christian Preschool | 122 Highland Avenue, Daytona Beach, FL 32117 | Center |
| Annette's Tender Loving Day Care | 1223 Center Avenue, Daytona Beach, FL 32117 | Preschool |
| Aunt Melinda's House | 955 Herbert Street, Port Orange, FL 32129 | Preschool |
| Bright Beginnings Academy | 910 Beville Road, Daytona Beach, FL 32114 | Preschool |
| Bright Beginnings Pre-School | 840 Taylor Road, Port Orange, FL 32127 | Center |
| Building Blocks Learning Academy | 228 Mason Avenue, Daytona Beach, FL 32117 | Center |
| C Kids Learning Academy | 829 Canal Street, New Smyrna Beach, FL 32168 | Preschool |
| Children's Country Club | 515 N Ridgewood Avenue, Daytona Beach, FL 32114 | Preschool |
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| Commonwealth Christian Academy | 5231 South Nova Road, Port Orange, FL 32127 | Preschool |
| Cor Meth Day School | 201 S Peninsula Avenue, New Smyrna Beach, FL 32169 | Preschool |
| Country Day School | 109 Dixwood Avenue, Edgewater, FL 32132 | Center |
| Daytona Beach Christian School | 101 Indigo Drive, Daytona Beach, FL 32114 | Preschool |
| Daytona Beach Leisure Services @ Turie T Sma | 800 South St, Daytona Beach, FL 32114 | Center |
| Daytona State College Child Development Center | 1200 W International Speedway B ..., Daytona Beach, FL 32120 | Preschool |
| Discovery Days Institute | 227 N Ridgewood Ave, Edgewater, FL 32132 | Preschool |
| Early Childhood Development Center | 3745 Nova Rd, Port Orange, FL 32129 | Center |
| East Volusia Education Center II | 313 Henry Butts Dr, Daytona Beach, FL 32114 | Center |
| East Volusia Educational Center | 431 S. Keech Street, Daytona Beach, FL 32114 | Preschool |
| East Volusia Head Start Center I | 461 S Keech St, Daytona Beach, FL 32114 | Center |
| Easter Seals - Project Warm | 417 Daytona Street, Daytona Beach, FL 32114 | Center |
| Easter Seals Society of Volusia & Flagler County | 1219 Dunn Ave, Daytona Beach, FL 32114 | Preschool |
| Ellison Acres Preschool | 2020 Lake Drive, New Smyrna Beach, FL 32168 | Preschool |
| Family Worship Center Preschool | 4425 S Ridgewood Ave, Port Orange, FL 32127 | Preschool |
| First Baptist Church of South Daytona After School Care | 2197 Kenilworth Ave, Daytona Beach, FL 32119 | Center |
| First Church Preschool | 305 Dunlawton Ave, Port Orange, FL 32127 | Preschool |
| First Friends Child Care | 115 S Orange Street, New Smyrna Beach, FL 32168 | Preschool |
| First Smyrna Learning Center | 310 Douglas Street, New Smyrna Beach, FL 32168 | Preschool |
| Friendship Academy | 404 N. Charles Street, Daytona Beach, FL 32114 | Preschool |
| Glencoe Learning Center | 196 N. Glencoe Road, New Smyrna Beach, FL 32168 | Preschool |
| Holly Hill Head Start | 719 Walker Street, Daytona Beach, FL 32117 | Preschool |
| Imagination Station | 510 Dunlawton Ave, Port Orange, FL 32127 | Preschool |
| Imagination Station Montessori | 528 N. Peninsula Dr., Daytona Beach, FL 32118 | Preschool |
| Imagination Station Too | 528 N Peninsula Dr, Daytona Beach, FL 32118 | Preschool |
| J. E. Denson Learning Center | 759 Derbyshire, Daytona Beach, FL 32114 | Center |
| Kid City USA - South Daytona | 2700 S Ridgewood Ave, Daytona Beach, FL 32119 | Preschool |
| Lighthouse Day Care & Preschool | 1247 Center Avenue, Daytona Beach, FL 32117 | Preschool |
| Lil' Angels Childcare | 1087 Mason Avenue, Daytona Beach, FL 32117 | Center |
| Lilies of The Fields | 201 San Juan Avenue, Daytona Beach, FL 32114 | Preschool |
| Little Explorers Montessori | 410 N Ridgewood Avenue, Edgewater, FL 32132 | Preschool |
| Little Ones Montessori | 960 Rooster Rd, Daytona Beach, FL 32119 | Preschool |
| Living Faith Academy | 950 Derbyshire Road, Daytona Beach, FL 32117 | Preschool |
| Mary McLeod Bethune Community Center | 740 S Ridgewood Ave, Daytona Beach, FL 32114 | Preschool |
| Meadowwood Early Learning Center | 900 Meadowwood Street, New Smyrna Beach, FL 32168 | Preschool |
| Montessori Magic | 632 Herbert Street, Port Orange, FL 32129 | Preschool |
| Montessori School of Port Orange | 3749 Nova Road, Port Orange, FL 32129 | Preschool |
| Mt. Calvary Academy | 700 Bellevue Ave, Daytona Beach, FL 32114 | Preschool |
| My School Preschool | 411 N Ridgewood Ave, Edgewater, FL 32132 | Preschool |
| Nanny's Childhood Learning Center | 312 N Duss St, New Smyrna Beach, FL 32168 | Preschool |
| New Horizon Childcare & Learning Center | 322 Pierce Avenue, Daytona Beach, FL 32114 | Center |
| Oaks Academy | 813 Big Tree Rd, Daytona Beach, FL 32119 | Center |
| One By One Learning Center | 628 Mason Avenue, Daytona Beach, FL 32117 | Center |
| Ortona Playground | 1206 N Halifax Ave, Daytona Beach, FL 32118 | Center |
| Parkside Academy | 1034 Ridgewood Avenue # 6, Daytona Beach, FL 32117 | Preschool |
| Pioneer Trail Day School | 2379 Pioneer Trail, New Smyrna Beach, FL 32168 | Preschool |
| Port Orange Presbyterian Preschool | 4662 S Clyde Morris Blvd, Port Orange, FL 32129 | Preschool |
| Port Orange YMCA Latch Key | 4701 City Center Parkway, Port Orange, FL 32129 | Center |
| Riviera Preschool | 103 Desoto Drive, New Smyrna Beach, FL 32169 | Preschool |
| Rose Marie Bryon Children 's Center | 625 South Street, Daytona Beach, FL 32114 | Center |
| Sandcastle Learning Center | 4590 Clyde Morris Blvd, Port Orange, FL 32129 | Preschool |
| Sandcastle Learning Center HLC & T | 950 N. Williamson Boulevard, Daytona Beach, FL 32114 | Preschool |
| Sandcastle Learning Center HT & LT | 4010 S Nova Road, Port Orange, FL 32127 | Preschool |
| Shining Star Children's Learning Center | 825 Big Tree Road, Daytona Beach, FL 32119 | Preschool |
| Small Steps Christian Learning Center | 1715 Taylor Road, Port Orange, FL 32128 | Preschool |
| South Daytona Christian School | 2121 Kenilworth Ave, Daytona Beach, FL 32119 | Preschool |
| St Pauls Episcopal Pre-School | 1650 Live Oak Street, New Smyrna Beach, FL 32168 | Preschool |
| St Timothy's Learning Center | 381 N Lincoln St, Daytona Beach, FL 32114 | Preschool |
| Sunshine House Preschool | 109 E Park Ave, Edgewater, FL 32132 | Preschool |
| T L C Kids Care at Living Cornerstone Church | 1835 Taylor Road, Port Orange, FL 32128 | Center |
| Temple Israel Preschool | 1400 S Peninsula Drive, Daytona Beach, FL 32118 | Preschool |
| The Chiles Academy | 868 George W. Engram Boulevard, Daytona Beach, FL 32114 | Center |
| Trinity Academy & Preschool | 233 Oakridge Street, Daytona Beach, FL 32117 | Preschool |
| Turie T Small Head Start Center | 800 South Street, Daytona Beach, FL 32114 | Preschool |
| Upward Bound Child Development and Learning Center | 211 Bay Street, Daytona Beach, FL 32114 | Preschool |
| Venetian Bay UMC After School Program | 569 N Airport Road, New Smyrna Beach, FL 32168 | Center |
| Volusia VPK Specialists | 1122 Mary Mcleod Bethune Blvd, Daytona Beach, FL 32114 | Preschool |
| Warner Christian Academy Preschool | 1730 S Ridgewood Ave, Daytona Beach, FL 32119 | Preschool |
| Westside Head Start Center | 1210 Jimmy Ann Drive, Daytona Beach, FL 32117 | Center |
| Wonderland Day Center | 348 South Keech Street, Daytona Beach, FL 32114 | Preschool |
| Word and Praise Christian Learning Center | 955 Orange Ave Suite 130, Daytona Beach, FL 32114 | Preschool |